Structure of PDB 7tzz Chain A

Receptor sequence

>7tzzA (length=582) Species: 3702 (Arabidopsis thaliana)

TFISRFAPDQPRKGADILVEALERQGVETVFAYPGGASMEIHQALTRSSS
IRNVLPRHEQGGVFAAEGYARSSGKPGICIATSGPGATNLVSGLADALLD
SVPLVAITGQVTRRMIGTDAFQETPIVEVTRSITKHNYLVMDVEDIPRII
EEAFFLATSGRPGPVLVDVPKDIQQQLAIPNWEQAMRLPGYMSRMPKPPE
DSHLEQIVRLISESKKPVLYVGGGCLNSSDELGRFVELTGIPVASTLMGL
GSYPCDDELSLHMLGMHGTVYANYAVEHSDLLLAFGVRFDDRVTGKLEAF
ASRAKIVHIDIDSAEIGKNKTPHVSVCGDVKLALQGMNKVLENRAEELKL
DFGVWRNELNVQKQKFPLSFKTFGEAIPPQYAIKVLDELTDGKAIISTGV
GQHQMWAAQFYNYKKPRQWLSSGGLGAMGFGLPAAIGASVANPDAIVVDI
DGDGSFIMNVQELATIRVENLPVKVLLLNNQHLGMVMQWEDRFYKANRAH
TFLGDPAQEDEIFPNMLLFAAACGIPAARVTKKADLREAIQTMLDTPGPY
LLDVICPHQEHVLPMIPSGGTFNDVITEGDGR

3D structure

• PDB: 7tzz Structural basis of resistance to herbicides that target acetohydroxyacid synthase.
• Chain: A
• Resolution: 2.59 Å

Enzymatic activity

• Enzyme Commision number: 2.2.1.6: acetolactate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	D538 N565 H567	D453 N480 H482
BS02	FAD	A	R246 G307 G308 G309 T331 L332 M333 M348 L349 M351 H352 G371 R373 V378 D395 I396 D414 V415 M490 G508	R161 G222 G223 G224 T246 L247 M248 M263 L264 M266 H267 G286 R288 V293 D310 I311 D329 V330 M405 G423
BS03	6QL	A	M351 R377 W574 S653	M266 R292 W489 S568
BS04	AUJ	A	V485 G486 Q487 H488 G511 M513 G537 D538 G539 S540 L568 G569 M570 V571	V400 G401 Q402 H403 G426 M428 G452 D453 G454 S455 L483 G484 M485 V486
BS05	MG	A	M543 Q546	M458 Q461
BS06	MG	A	A122 T167 S168 Q207	A37 T82 S83 Q122
BS07	MG	A	Y466 K618	Y381 K533

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003824 catalytic activity
• GO:0003984 acetolactate synthase activity
• GO:0030976 thiamine pyrophosphate binding
• GO:0050660 flavin adenine dinucleotide binding

Biological Process

• GO:0009082 branched-chain amino acid biosynthetic process

External links

• PDB: RCSB:7tzz, PDBe:7tzz, PDBj:7tzz
• PDBsum: 7tzz
• PubMed: 35690625
• UniProt: P17597|ILVB_ARATH Acetolactate synthase, chloroplastic (Gene Name=ALS)