Structure of PDB 7tfi Chain A

Receptor sequence
>7tfiA (length=391) Species: 4932 (Saccharomyces cerevisiae) [Search protein sequence]
REEDKLWTVKYAPTNLQQVCGNKGSVMKLKNWLANWENSKKNSFKHAGKD
GSGVFRAAMLYGPPGIGKTTAAHLVAQELGYDILEQNASDVRSKTLLNAG
VKNALDNMSVVGYFKHQNLNGKHFVIIMDEVDGMGGVGQLAQFCRKTSTP
LILICNERNLPKMRPFDRVCLDIQFRRPDANSIKSRLMTIAIREKFKLDP
NVIDRLIQTTRGDIRQVINLLSTISTTTKTINHENINEISKAWEKNIALK
PFDIAHKMLDGQIYSDIGSRNFTLNDKIALYFDDFDFTPLMIQENYLSTR
PSVLKPGQSHLEAVAEAANCISLGDIVEKKIRSSEQLWSLLPLHAVLSSV
YPASKVAGHMAGRINFTAWLGQNSKSAKYYRLLQEIHYHTR
3D structure
PDB7tfi Cryo-EM structures reveal that RFC recognizes both the 3'- and 5'-DNA ends to load PCNA onto gaps for DNA repair.
ChainA
Resolution3.41 Å
3D
structure
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Enzymatic activity
Enzyme Commision number ?
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A F587 R632 Q636 L670 F287 R332 Q336 L370
BS02 dna A F582 W638 S639 F282 W338 S339
BS03 AGS A T299 A303 C311 P355 G356 I357 G358 K359 T360 T361 N456 I514 R515 T8 A12 C20 P64 G65 I66 G67 K68 T69 T70 N156 I214 R215
BS04 MG A T360 D424 T69 D129
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003689 DNA clamp loader activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016887 ATP hydrolysis activity
Biological Process
GO:0000278 mitotic cell cycle
GO:0006260 DNA replication
GO:0006261 DNA-templated DNA replication
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006272 leading strand elongation
GO:0006281 DNA repair
GO:0006298 mismatch repair
GO:0051301 cell division
Cellular Component
GO:0005634 nucleus
GO:0005663 DNA replication factor C complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7tfi, PDBe:7tfi, PDBj:7tfi
PDBsum7tfi
PubMed35829698
UniProtP38630|RFC1_YEAST Replication factor C subunit 1 (Gene Name=RFC1)

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