Structure of PDB 7t29 Chain A

Receptor sequence

>7t29A (length=453) Species: 287 (Pseudomonas aeruginosa)

HHHHHHMQLSSLTAVSPVDGRYAGKTSSLRPIFSEYGLIRFRVMVEVRWL
QRLAAHAGIPEVAPFSAEANALLDSLASDFQLEHAERIKEIERTTNHDVK
AVEYLLKEQAAKLPELAAVSEFIHFACTSEDINNLSHALMLREGRDSVLL
PLMRQIAEAIRELAVKLADVPMLSRTHGQPASPTTLGKELANVVYRLERQ
IKQVAGIELLGKINGAVGNYNAHLSAYPEVDWEANARQFIEGDLGLTFNP
YTTQIEPHDYIAELFDAIARFNTILIDFDRDVWGYISLGYFKQKTVAGEI
GVNPIDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGI
AHSIIAYEASLKGIGKLELNAQRIAEDLDACWEVLAEPVQTVMRRYGVEN
PYEKLKELTRGISAEALQTFIEELAIPAEAKVELKKLTPAGYVGNAAAQA
KRI

3D structure

• PDB: 7t29 Crystal Structure of Adenylosuccinate lyase (ASL) from Pseudomonas aeruginosa complexed with AMP
• Chain: A
• Resolution: 1.5 Å

Enzymatic activity

• Enzyme Commision number: 4.3.2.2: adenylosuccinate lyase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AMP	A	H91 D92 Q248 R336 L338 S341 T342 R345	H97 D98 Q254 R335 L337 S340 T341 R344

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004018 N6-(1,2-dicarboxyethyl)AMP AMP-lyase (fumarate-forming) activity
• GO:0016829 lyase activity
• GO:0070626 (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido) succinate lyase (fumarate-forming) activity

Biological Process

• GO:0006164 purine nucleotide biosynthetic process
• GO:0006188 IMP biosynthetic process
• GO:0006189 'de novo' IMP biosynthetic process
• GO:0009152 purine ribonucleotide biosynthetic process
• GO:0044208 'de novo' AMP biosynthetic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:7t29, PDBe:7t29, PDBj:7t29
• PDBsum: 7t29
• UniProt: Q9I0K9|PUR8_PSEAE Adenylosuccinate lyase (Gene Name=purB)