Structure of PDB 7t1b Chain A

Receptor sequence

>7t1bA (length=438) Species: 4932 (Saccharomyces cerevisiae)

IVACDDPDFLTSYFAHSRLHHLSAWKANLKDKFLNENIHKYTKITDKDTY
IIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVCHGTKNSDIASCNYV
ARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYSTLKRLN
IFNLILPISIDEAVCVRIIPDNIHNTNTLNARLCEEIRQEIFQGTNGCTV
SIGCSDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGHS
TLSRLESTFDSPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDEE
SLKILYDPKEVLQRKSLSIDINWGIRFKNITQVDLFIERGCQYLLEKLNE
INKTTSQITLKLMRRCKDAPIEPPKYMGMGRCDSFSRSSRLGIPTNEFGI
IATEMKSLYRTLGCPPMELRGLALQFNKLVDVGPDNNQ

3D structure

• PDB: 7t1b Mechanism of nucleotide discrimination by the translesion synthesis polymerase Rev1.
• Chain: A
• Resolution: 1.75 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	I464 S465 E468 P551 G552 G554 S556 T557 Q663 S692 R693 S694 R696	I158 S159 E162 P245 G246 G248 S250 T251 Q357 S386 R387 S388 R390
BS02	dna	A	Y319 F320 H322 S323 R324 L325 H326 S329 H393 G394 T395 K396 N397 S398 D399 K590 R620 K621 S624 I625 D626 N628 W629 K681 Y682 G684 M685 G686	Y13 F14 H16 S17 R18 L19 H20 S23 H87 G88 T89 K90 N91 S92 D93 K284 R314 K315 S318 I319 D320 N322 W323 K375 Y376 G378 M379 G380
BS03	CTP	A	R324 L328 D362 F363 D364 C365 F367 A401 Y405 R408 N414 D467 K525	R18 L22 D56 F57 D58 C59 F61 A95 Y99 R102 N108 D161 K219
BS04	CA	A	D362 F363 D467	D56 F57 D161
BS05	CA	A	D362 D467 E468	D56 D161 E162

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7t1b, PDBe:7t1b, PDBj:7t1b
• PDBsum: 7t1b
• PubMed: 35610266
• UniProt: P12689|REV1_YEAST DNA repair protein REV1 (Gene Name=REV1)