Structure of PDB 7t18 Chain A

Receptor sequence

>7t18A (length=439) Species: 4932 (Saccharomyces cerevisiae)

RIVACDDPDFLTSYFAHSRLHHLSAWKANLKDKFLNENIHKYTKITDKDT
YIIFHIDFDCFFATVAYLCRSSSFSACDFKRDPIVVCHGTKNSDIASCNY
VARSYGIKNGMWVSQAEKMLPNGIKLISLPYTFEQFQLKSEAFYSTLKRL
NIFNLILPISIDEAVCVRIIPDNIHNTNTLNARLCEEIRQEIFQGTNGCT
VSIGCSDSLVLARLALKMAKPNGYNITFKSNLSEEFWSSFKLDDLPGVGH
STLSRLESTFDSPHSLNDLRKRYTLDALKASVGSKLGMKIHLALQGQDDE
ESLKILYDPKEVLQRKSLSIDINWGIRFKNITQVDLFIERGCQYLLEKLN
EINKTTSQITLKLMRRCKDAPIEPPKYMGMGRCDSFSRSSRLGIPTNEFG
IIATEMKSLYRTLGCPPMELRGLALQFNKLVDVGPDNNQ

3D structure

• PDB: 7t18 Mechanism of nucleotide discrimination by the translesion synthesis polymerase Rev1.
• Chain: A
• Resolution: 1.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L328 I464 S465 E468 R518 G552 G554 H555 S556 T557 S692 R693 S694 R696	L23 I159 S160 E163 R213 G247 G249 H250 S251 T252 S387 R388 S389 R391
BS02	dna	A	Y319 F320 H322 S323 R324 L325 H326 S329 H393 G394 T395 K396 N397 S398 D399 K590 R620 K621 S622 S624 I625 D626 N628 W629 K681 Y682 G684 M685 G686	Y14 F15 H17 S18 R19 L20 H21 S24 H88 G89 T90 K91 N92 S93 D94 K285 R315 K316 S317 S319 I320 D321 N323 W324 K376 Y377 G379 M380 G381
BS03	CA	A	D362 F363 D467	D57 F58 D162
BS04	CA	A	D362 D467 E468	D57 D162 E163
BS05	CA	A	D548 L550 V553	D243 L245 V248
BS06	TTP	A	R324 L328 D362 D364 C365 F366 F367 S402 Y405 R408 N414 D467 K525	R19 L23 D57 D59 C60 F61 F62 S97 Y100 R103 N109 D162 K220

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7t18, PDBe:7t18, PDBj:7t18
• PDBsum: 7t18
• PubMed: 35610266
• UniProt: P12689|REV1_YEAST DNA repair protein REV1 (Gene Name=REV1)