Structure of PDB 7shl Chain A

Receptor sequence

>7shlA (length=645) Species: 8355 (Xenopus laevis)

SLKPRVVDFDETWNKLLTTIKAVVMLDYVERATWNDRFSDIYALCVAYPE
PLGERLYTETKIFLENHVQQLHTRVLDSAEQVLVMYFRYWEEYSRGADYM
DCLYRYLNTQYIKKNKLTEADLQYGYGGVDMNEPLMEIGELALDLWRKLM
IEPLQDTLLIMLLREIKRDRCGEDPNQKVIHGVINSFVHVEQYKKKFPLK
FYQEIFESPFLAETGEYYKQEASNLLQESNCSQYMEKILGRLKDEEIRCR
KYLHPSSYNKVIHECQQRMVADHLQFLHAECHNIIRQERRNDMANMYTLL
RAVSSGLPHMIQELQNHIHDEGLRAISNLSQENMPTQFVESVLEVHSKFV
QLVNCVLNGDQHFMSALDKALTCVVNYREPKSVCKAPELLAKYCDNMLKK
SAKGMTENEVEDKLTSFITVFKYIDDKDVFQKFYARMLAKRLIHGLSMSM
DSEETMINKLKQACGYEFTSKLHRMYTDMSVSADLNNKFNNFIKSQDTVI
DLGISFQIYVLQAGAWPLTQAPSSTFAIPQELEKSVQMFELFYNQHFSGR
KLTWLHYLCTGEVKMNYLCKPYVAMVTTYQMAVLLAFNNSEIITYKELQD
STQMNEKELTKTIKSLLDVKMINHDSDKEDIEGESTFSLNMNFSS

3D structure

• PDB: 7shl Structure of CRL2Lrr1, the E3 ubiquitin ligase that promotes DNA replication termination in vertebrates.
• Chain: A
• Resolution: 3.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	G504 I505 S506 F507 Q508 I509 Y510 V511 L512 Q513 A514 L533 Y544 G550 R551 K552 L553 T554 L556 C560 T561 G562 E563 K565 M566 Y568 L569 M582 N589	G503 I504 S505 F506 Q507 I508 Y509 V510 L511 Q512 A513 L532 Y543 G549 R550 K551 L552 T553 L555 C559 T560 G561 E562 K564 M565 Y567 L568 M581 N588

Gene Ontology

Molecular Function

• GO:0030674 protein-macromolecule adaptor activity
• GO:0031625 ubiquitin protein ligase binding

Biological Process

• GO:0006511 ubiquitin-dependent protein catabolic process
• GO:0016567 protein ubiquitination
• GO:0031146 SCF-dependent proteasomal ubiquitin-dependent protein catabolic process

Cellular Component

• GO:0019005 SCF ubiquitin ligase complex
• GO:0031461 cullin-RING ubiquitin ligase complex
• GO:0031462 Cul2-RING ubiquitin ligase complex

External links

• PDB: RCSB:7shl, PDBe:7shl, PDBj:7shl
• PDBsum: 7shl
• PubMed: 34850944
• UniProt: Q6PCF2