Structure of PDB 7s4x Chain A

Receptor sequence
>7s4xA (length=1354) Species: 1314 (Streptococcus pyogenes) [Search protein sequence]
KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILR
VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKN
GYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFL
DNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYT
GWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKED
IQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIE
MARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDK
NRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELD
KAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKL
VSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGD
YKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPL
IETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILP
KRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKE
LLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKR
MLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQH
KHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHL
FTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDL
SQLG
3D structure
PDB7s4x Structural basis for mismatch surveillance by CRISPR-Cas9.
ChainA
Resolution2.76 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 rna A K30 K33 L35 I43 K44 N46 G56 E57 T58 A59 T62 R63 K65 R66 R69 R70 R71 Y72 R74 R75 K76 L101 S104 F105 K111 R115 H116 I122 E125 H129 T134 I135 H160 K163 F164 R165 G166 H167 Y325 H328 H329 L332 K336 R340 K348 F351 F352 Y359 I363 Q402 R403 T404 F405 N407 G408 F446 R447 I448 Y450 G453 P454 L455 A456 R457 N459 S460 F462 W464 T472 I473 F491 K510 Y515 T519 N588 R661 M694 H721 S730 P731 K735 L738 K749 S777 R778 E779 R780 N803 Q807 K848 D849 D850 S851 K918 R919 V922 Y981 S1006 V1009 Y1010 G1011 Y1013 Y1016 K1031 P1090 Q1091 V1100 T1102 G1103 G1104 F1105 I1110 P1112 K1113 R1122 K1123 K1124 K1130 Y1131 M1169 E1225 A1227 Q1272 H1349 Q1350 S1351 Y1356 K28 K31 L33 I41 K42 N44 G54 E55 T56 A57 T60 R61 K63 R64 R67 R68 R69 Y70 R72 R73 K74 L99 S102 F103 K109 R113 H114 I120 E123 H127 T132 I133 H158 K161 F162 R163 G164 H165 Y318 H321 H322 L325 K329 R333 K341 F344 F345 Y352 I356 Q395 R396 T397 F398 N400 G401 F439 R440 I441 Y443 G446 P447 L448 A449 R450 N452 S453 F455 W457 T465 I466 F484 K503 Y508 T512 N581 R654 M687 H709 S718 P719 K723 L726 K737 S765 R766 E767 R768 N791 Q795 K836 D837 D838 S839 K906 R907 V910 Y969 S994 V997 Y998 G999 Y1001 Y1004 K1019 P1078 Q1079 V1088 T1090 G1091 G1092 F1093 I1098 P1100 K1101 R1110 K1111 K1112 K1118 Y1119 M1157 E1213 A1215 Q1260 H1337 Q1338 S1339 Y1344
BS02 dna A N863 R864 D868 K1107 E1108 S1109 N851 R852 D856 K1095 E1096 S1097
BS03 dna A Y450 I492 M495 T496 N497 G582 E584 E627 R655 T657 W659 R661 R765 E766 Q768 T770 G773 Q774 K810 D837 V838 H840 Q844 R864 R925 Q926 K929 Y1016 D1017 V1018 A1032 Y443 I485 M488 T489 N490 G575 E577 E620 R648 T650 W652 R654 R753 E754 Q756 T758 G761 Q762 K798 D825 V826 H828 Q832 R852 R913 Q914 K917 Y1004 D1005 V1006 A1020
BS04 dna A G12 T13 N14 S15 G915 F916 Q920 K974 R976 H983 Y1001 K1003 L1004 E1007 R1019 F1037 F1038 N1041 I1042 R1058 S1116 K1118 D1135 S1136 K1200 S1216 Q1221 A1320 R1333 R1335 G10 T11 N12 S13 G903 F904 Q908 K962 R964 H971 Y989 K991 L992 E995 R1007 F1025 F1026 N1029 I1030 R1046 S1104 K1106 D1123 S1124 K1188 S1204 Q1209 A1308 R1321 R1323
BS05 MG A D10 E762 D8 E750
BS06 MG A D10 H983 D8 H971
Gene Ontology
Molecular Function
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003723 RNA binding
GO:0004519 endonuclease activity
GO:0004520 DNA endonuclease activity
GO:0004527 exonuclease activity
GO:0008408 3'-5' exonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0043571 maintenance of CRISPR repeat elements
GO:0051607 defense response to virus

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Molecular Function
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Biological Process
External links
PDB RCSB:7s4x, PDBe:7s4x, PDBj:7s4x
PDBsum7s4x
PubMed35236982
UniProtQ99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)

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