Structure of PDB 7s4v Chain A

Receptor sequence

>7s4vA (length=1122) Species: 1314 (Streptococcus pyogenes)

KKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALL
FDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLE
ESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLR
LIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPIN
ASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNF
KSNFDLAEDAKLQLSKDTYDDDLDNLLAQIDLFLAAKNLSDAILLSDILR
VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKN
GYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDN
GSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLAR
GNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKV
LPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNR
KVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFL
DNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYT
GWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKED
IQKAQDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIE
MARENRQITKHVAQILDSRMNTKYDENDKIREVKVITLKSKLVSDFRKDF
QFYKVREINNYHHAHDAYLNAVVGTALIKKYKGRDFATVRKVLSMPQVNI
VKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVL
VVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDL
IIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEK
LKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAY
NKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLG

3D structure

• PDB: 7s4v Structural basis for mismatch surveillance by CRISPR-Cas9.
• Chain: A
• Resolution: 3.28 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K33 K44 N46 E57 T58 A59 T62 R63 K65 R69 R70 R74 N77 L101 S104 F105 R115 H129 T134 R165 G166 Y325 H328 H329 R340 F351 F352 I363 Q402 R403 T404 F405 N407 G408 F446 I448 A456 R457 N459 R467 I473 Y515 N588 R661 M694 K929 P1090 E1099 V1100 F1105 I1110 R1122 K1124 K1130 Y1131 M1169 A1227 H1349 Q1350 S1351	K31 K42 N44 E55 T56 A57 T60 R61 K63 R67 R68 R72 N75 L99 S102 F103 R113 H127 T132 R163 G164 Y318 H321 H322 R333 F344 F345 I356 Q395 R396 T397 F398 N400 G401 F439 I441 A449 R450 N452 R460 I466 Y508 N581 R654 M687 K760 P846 E855 V856 F861 I866 R878 K880 K886 Y887 M925 A983 H1105 Q1106 S1107
BS02	dna	A	Y450 M495 N497 K526 K528 G582 E584 Q695 K1107 E1108 S1109	Y443 M488 N490 K519 K521 G575 E577 Q688 K863 E864 S865
BS03	dna	A	R1114 K1118 S1216 Q1221	R870 K874 S972 Q977

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0004520 DNA endonuclease activity
• GO:0004527 exonuclease activity
• GO:0008408 3'-5' exonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0043571 maintenance of CRISPR repeat elements
• GO:0051607 defense response to virus

External links

• PDB: RCSB:7s4v, PDBe:7s4v, PDBj:7s4v
• PDBsum: 7s4v
• PubMed: 35236982
• UniProt: Q99ZW2|CAS9_STRP1 CRISPR-associated endonuclease Cas9/Csn1 (Gene Name=cas9)