Structure of PDB 7r3x Chain A

Receptor sequence

>7r3xA (length=1122) Species: 4932 (Saccharomyces cerevisiae)

KYNTLSNNYALSAQQLLNASKIDDIDSMMGFERYVPPQYNGRFDAKDIDQ
IPGRVGWLTNMHATLVSQENQGISGVDFYFLDEEGGSFKSTVVYDPYFFI
ACNDESRVNDVEELVKKYLESCLKSLQIIRKEDLTMDNHLLGLQKTLIKL
SFVNSNQLFEARKLLRPILQDNANNNVQRNIYNVKVDAKHLIEDIREYDV
PYHVRVSIDKDIRVGKWYKVTQQGFIEDTRKIAFADPVVMAFDIETTKPP
LKFPDSAVDQIMMISYMIDGEGFLITNREIISEDIEDFEYTPKPEYPGFF
TIFNENDEVALLQRFFEHIRDVRPTVISTFNGDFFDWPFIHNRSKIHGLD
MFDEIGFAPDAEGEYKSSYCSHMDCFRWVKRDSYLPQGSQGVKAVTQSKL
GYNPIELDPELMTPYAFEKPQHLSEYSVSDAVATYYLYMKYVHPFIFSLC
TIIPLNPDETLRKGTGTLCEMLLMVQAYQHNILLPNKHTDPIERFYDGHL
LESETYVGGHVESLEAGVFRSDLKNEFKIDPSAIDELLQELPEALKFSVE
VENKSSVDKVTNFEEIKNQITQKLLELKENNIRNELPLIYHVDVASMYPN
IMTTNRLQPDSIKTCARKLKWAWRGEFFPSKMDEYNMIKRALQNETFPNK
NKFSKKKVLTFDELSYADQVIHIKKRLTEYSRKVYHRVKVSEIVEREAIV
CQRENPFYVDTVKSFRDRRYEFKGLAKTWKGNLSKIDPSDKHARDEAKKM
IVLYDSLQLAHKVILNSFYGYVMRKGSRWYSMEMAGITCLTGATIIQMAR
ALVERVGRPLELDTDGIWCILPKSFPETYFFTLENGKKLYLSYPCSMLNY
RVHQKFTNHQYQELKDPLNYIYETHSENTIFFEVDGPYKAMILPSSKEEG
KGIKKRYAVFNEDGSLAELKGFELKRRGELQLIKNFQSDIFKVFLEGDTL
EGCYSAVASVCNRWLDVLDSHGLMLEDEDLVSLICENRSMSKTLKEYEGQ
KSTSITTARRLGDFLGEDMVKDKGLQCKYIISSKPFNAPVTERAIPVAIF
SADIPIKRSFLRRWTLDPSLEDLDIRTIIDWGYYRERLGSAIQKIITIPA
ALQGVSNPVPRVEHPDWLKRKI

3D structure

• PDB: 7r3x Enhanced polymerase activity permits efficient synthesis by cancer-associated DNA polymerase ε variants at low dNTP levels.
• Chain: A
• Resolution: 2.46 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  3.1.11.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	P433 Q434 D875 K967 Y969 K982 G983 K987 R988 R989 S1051 S1053 Y1059	P386 Q387 D813 K905 Y907 K920 G921 K925 R926 R927 S989 S991 Y997
BS02	dna	A	K510 G511 T512 G513 T514 T552 Y553 G555 G556 V558 R686 S829 G832 M835 R836 K837 I965 K966 K967 K1063 T1103 Y1145 K1156	K463 G464 T465 G466 T467 T505 Y506 G508 G509 V511 R624 S767 G770 M773 R774 K775 I903 K904 K905 K1001 T1041 Y1083 K1094
BS03	DTP	A	D640 V641 S643 M644 Y645 R781 K824 N828 D877	D593 V594 S596 M597 Y598 R719 K762 N766 D815
BS04	CA	A	D640 V641 D877	D593 V594 D815
BS05	CA	A	D290 E292 D477	D243 E245 D430

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006260 DNA replication
• GO:0006281 DNA repair

Cellular Component

• GO:0008622 epsilon DNA polymerase complex

External links

• PDB: RCSB:7r3x, PDBe:7r3x, PDBj:7r3x
• PDBsum: 7r3x
• PubMed: 35822874
• UniProt: P21951|DPOE_YEAST DNA polymerase epsilon catalytic subunit A (Gene Name=POL2)