Structure of PDB 7qw5 Chain A

Receptor sequence

>7qw5A (length=544) Species: 1422 (Geobacillus stearothermophilus) [Search protein sequence]

VSRQKATGAHFTPDKLAEVIAKRILDYFKGEKNRVIRVLDPACGDGELLL
AINKVAQSMNIQLELIGVDFDIDAINIANERLSRSGHKNFRLINKDFLEM
LEPVDIIIANPPYVRTQILGAEKAQKLREKFNLKGRVDLYQAFLVAMTQQ
LKSNGIIGVITSNRYLTTKGGESTRKFLVSNFNILEIMDLGDSKFFEAAV
LPAIFFGEKKNKESNVPKFFKIYEQSDIEASSSVNSEFNSLIELLEVNKS
GLYSVEDKTYSISLGKIISPENYKEPWILATEDEYEWFMKVNQNAYGFIE
DFAHVKVGIKTTADSVFIRSDWGELPEEQIPEDKLLRPIISADQANKWSV
SGNNKKVLYTHEIRDGQIKAINLEEFPRAKNYLESHKERLASRKYVLKAN
RNWYEIWVPHDPSLWDKPKIIFPDTSPEPKFFYEDKGSVVDGNCYWIIPK
KENSNDILFLIMGICNSKFMSKYHDIAFQNKLYAGRRRYLTQYVNKYPIP
DPESIYSKEIISLVRELVNNETQDINEIENRIEKLILRAFDIES

3D structure

PDB

7qw5 Structure of M.BseCI DNA methyltransferase from Geobacillus stearothermophilus.

Chain

Resolution

2.3 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	K14 G17 H19 N133 P134 Y136 R138 R159 R187 K192 G193 F219 A221 A222 K334 V335 H440 Y475 L512 Y513 R518 T521 Q522	K5 G8 H10 N110 P111 Y113 R115 R136 R164 K169 G170 F196 A198 A199 K306 V307 H410 Y445 L482 Y483 R488 T491 Q492
BS02	dna	A	T139 Q140 A144 R151 G158 R159 K338 T340 A341 D342 Y425 W437 N473 L512 Y513 R516	T116 Q117 A121 R128 G135 R136 K310 T312 A313 D314 Y395 W407 N443 L482 Y483 R486
BS03	SAH	A	T16 G17 A18 H19 F20 T21 D49 A51 C52 G53 L57 D78 F79 D105 F106 N133 P135 F166	T7 G8 A9 H10 F11 T12 D40 A42 C43 G44 L48 D69 F70 D96 F97 N110 P112 F143

Gene Ontology

	Molecular Function
GO:0003676	nucleic acid binding
GO:0003677	DNA binding
GO:0008168	methyltransferase activity
GO:0009007	site-specific DNA-methyltransferase (adenine-specific) activity

	Biological Process
GO:0006304	DNA modification
GO:0009307	DNA restriction-modification system
GO:0032259	methylation

View graph for
Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:7qw5, PDBe:7qw5, PDBj:7qw5
PDBsum	7qw5
PubMed
UniProt	P43423\|MTC1_GEOSE Type II methyltransferase M.BseCI (Gene Name=bseCIM)

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