Structure of PDB 7qh1 Chain A

Receptor sequence

>7qh1A (length=298) Species: 287 (Pseudomonas aeruginosa)

AEAGGPGGNQKIGKYTYGSDYGPLIVNDRCEMDDGNVITVDMNSSTDDSK
TTPFRFACPTNTYKQVNGAYSPLNDAHFFGGVVFKLYRDWFGTSPLTHKL
YMKVHYGRSVENAYWDGTAMLFGDGATMFYPLVSLDVAAHEVSHGFTEQN
SGLIYRGQSGGMNEAFSDMAGEAAEFYMRGKNDFLIGYDIKKGSGALRYM
DQPSRDGRSIDNASQYYNGIDVHHSSGVYNRAFYLLANSPGWDTRKAFEV
FVDANRYYWTATSNYNSGACGVIRSAQNRNYSAADVTRAFSTVGVTCP

3D structure

• PDB: 7qh1 Chemical Optimization of Selective Pseudomonas aeruginosa LasB Elastase Inhibitors and Their Impact on LasB-Mediated Activation of IL-1 beta in Cellular and Animal Infection Models.
• Chain: A
• Resolution: 2.74 Å

Enzymatic activity

• Enzyme Commision number: 3.4.24.26: pseudolysin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	H140 H144 E164	H140 H144 E164
BS02	CI8	A	E111 N112 M128 F129 V137 H140 E141 H144 E164 I186 G187 I190 L197 R198 R208 H223	E111 N112 M128 F129 V137 H140 E141 H144 E164 I186 G187 I190 L197 R198 R208 H223
BS03	CA	A	D136 E172 E175 D183 L185	D136 E172 E175 D183 L185
BS04	CA	A	Y217 H224	Y217 H224

Gene Ontology

Molecular Function

• GO:0004222 metalloendopeptidase activity

Biological Process

• GO:0006508 proteolysis

External links

• PDB: RCSB:7qh1, PDBe:7qh1, PDBj:7qh1
• PDBsum: 7qh1
• PubMed: 36669138
• UniProt: P14756|ELAS_PSEAE Elastase (Gene Name=lasB)