Structure of PDB 7qbv Chain A

Receptor sequence

>7qbvA (length=411) Species: 2214 (Methanosarcina acetivorans)

MEVVVDVGGNPGVDCKGFCKYCYFKKVKDIQPLGCKYCLPFKKGCDYCTR
SVKESYSGFKSLQMVLEETANKLYFTSGEVKKFTVSGGGDLSCYPELKSL
ITFLSQFNTPIHLGYTSGKGFSKPDDALFYIDNGVTEVSFTVFATDPALR
AEYMKDPEPEASIQVLRDFCTHCEVYGAIVLLPGINDGEVLEKTLCDLEN
MGAKGAILMRFANFQENGLILNNSPIIPGITPHTVSEFTEIVRSSAEKHP
SIRITGTPLEDPLIGSPFAIRNVPEALLKLPRVSKKATIITGQVAASRLT
EIFEALGGTVNVIPVKKDIGCLITIDDFKALDLSEVTETVFIPGRAFVHD
MEIKEALRRDGVDRIVRRGPERLSVDGEMSIGMTREEVLELEVENFTELI
GQINSLGLPLE

3D structure

• PDB: 7qbv Crystallographic snapshots of a B 12 -dependent radical SAM methyltransferase.
• Chain: A
• Resolution: 2.701 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.379: [methyl coenzyme M reductase]-L-arginine C-5-methyltransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	A	P11 G12 C15 F18 C19 C22 Y115 S117 K119	P11 G12 C15 F18 C19 C22 Y115 S117 K119
BS02	FE	A	C35 C38 C45 C48	C35 C38 C45 C48
BS03	SAH	A	Y21 Y23 G87 Y115 V180 M209 F211 A212	Y21 Y23 G87 Y115 V180 M209 F211 A212
BS04	COB	A	Y23 F24 Y47 R210 A212 N217 G218 L219 L221 N223 P258 P267 F268 I289 T291 D318 I319 G320 C321 L322 F341 I342 P343 R345 F347 G369 P370 E371 R372 L373 S374 D376 E378	Y23 F24 Y47 R210 A212 N217 G218 L219 L221 N223 P258 P267 F268 I289 T291 D318 I319 G320 C321 L322 F341 I342 P343 R345 F347 G369 P370 E371 R372 L373 S374 D376 E378

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0008168 methyltransferase activity
• GO:0035244 protein-arginine C-methyltransferase activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0032259 methylation
• GO:0043687 post-translational protein modification

External links

• PDB: RCSB:7qbv, PDBe:7qbv, PDBj:7qbv
• PDBsum: 7qbv
• PubMed: 35110733
• UniProt: Q8THG6|MCRAM_METAC [Methyl coenzyme M reductase]-L-arginine C-5-methyltransferase (Gene Name=MA_4551)