Structure of PDB 7pwg Chain A

Receptor sequence

>7pwgA (length=249) Species: 184922 (Giardia lamblia ATCC 50803)

GRRIRVQRKGAGSVFRAHVARRLGAAKLRANDFAERTGSVRGVVKAILHD
RGRGAPLARVQFPNMRGEGRVNELFIAPEGMYTGQEVFSGNKATLHIGNI
LPVGNIPEGTYVCNVEEKPMDRGCLARASGTYCLVVAHNMETNKTRIRLP
SGQKKLISSKARAMIGLVAGGGRTDKPLLKAGNAYHKYKAKRNCWPVVRG
VAMNPVDHPHGGGNHQHIGVSSCVPRNAVPGQKVGLIAARRTGCSGGKR

3D structure

• PDB: 7pwg Cryo-EM structure of the ancient eukaryotic ribosome from the human parasite Giardia lamblia.
• Chain: A
• Resolution: 2.75 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	G2 R3 R4 V7 Q8 R9 G11 S14 A18 H19 V20 R23 G25 A26 D33 F34 R37 H50 R54 Q62 R67 G70 R71 E118 K119 M121 R123 C125 L126 A127 R128 A129 S130 L150 S152 Q154 K156 R174 K177 L179 K181 A182 G183 H187 R193 W196 P197 V198 V199 R200 G201 V202 A203 M204 N205 V207 H211 G214 N215 H216 H218 G220 V221 S222 S223 C224 P226 R227 N228 V230 P231 G232 Q233 K234 V235 G236 I238 A239 A240 R241 T243 G244 C245	G1 R2 R3 V6 Q7 R8 G10 S13 A17 H18 V19 R22 G24 A25 D32 F33 R36 H49 R53 Q61 R66 G69 R70 E117 K118 M120 R122 C124 L125 A126 R127 A128 S129 L149 S151 Q153 K155 R173 K176 L178 K180 A181 G182 H186 R192 W195 P196 V197 V198 R199 G200 V201 A202 M203 N204 V206 H210 G213 N214 H215 H217 G219 V220 S221 S222 C223 P225 R226 N227 V229 P230 G231 Q232 K233 V234 G235 I237 A238 A239 R240 T242 G243 C244

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0003735 structural constituent of ribosome

Biological Process

• GO:0002181 cytoplasmic translation
• GO:0006412 translation

Cellular Component

• GO:0005840 ribosome
• GO:0022625 cytosolic large ribosomal subunit
• GO:0022626 cytosolic ribosome
• GO:1990904 ribonucleoprotein complex

External links

• PDB: RCSB:7pwg, PDBe:7pwg, PDBj:7pwg
• PDBsum: 7pwg
• PubMed: 35100413
• UniProt: E2RTN4