Structure of PDB 7pem Chain A

Receptor sequence

>7pemA (length=1066) Species: 559292 (Saccharomyces cerevisiae S288C)

PRVIHINDSLANSSFGYSDNHISTTKYNFATFLPKFLFQEFSKYANLFFL
CTSAIQQVPHVSPTNRYTTIGTLLVVLIVSAMKECIEDIKRANSDKELNN
STAEIFSEAHDDFVEKRWIDIRVGDIIRVKSEEPIPADTIILSSSEPEGL
CYIETANLDGETNLKIKQSRVETAKFIDVKTLKNMNGKVVSEQPNSSLYT
YEGTMTLNDRQIPLSPDQMILRGATLRNTAWIFGLVIFTGHETKLLRNAT
ATPIKRTAVEKIINRQIIALFTVLIVLILISSIGNVIMSTADAKHLSYLY
LEGTNKAGLFFKDFLTFWILFSNLVPISLFVTVELIKYYQAFMIGSDLDL
YYEKTDTPTVVRTSSLVEELGQIEYIFSDKTGTLTRNIMEFKSCSIAGHC
YIDKIPEDKTATVEDGIEVGYRKFDDLKKKLNDPSDEDSPIINDFLTLLA
TCHTVIPEFQSDGSIKYQAASPDEGALVQGGADLGYKFIIRKPNSVTVLL
EETGEEKEYQLLNICEFNSTRKRMSAIFRFPDGSIKLFCKGADTVILERL
DDEANQYVEATMRHLEDYASEGLRTLCLAMRDISEGEYEEWNSIYNEAAT
TLDNRAEKLDEAANLIEKNLILIGATAIEDKLQDGVPETIHTLQEAGIKI
WVLTGDRQETAINIGMSCRLLSEDMNLLIINEETRDDTERNLLEKINALN
EHQLSTHDMNTLALVIDGKSLGFALEPELEDYLLTVAKLCKAVICCRVSP
LQKALVVKMVKRKSSSLLLAIGDGANDVSMIQAAHVGVGISGMEGMQAAR
SADIAVGQFKFLKKLLLVHGSWSYQRISVAILYSFYKNTALYMTQFWYVF
ANAFSGQSIMESWTMSFYNLFFTVWPPFVIGVFDQFVSSRLLERYPQLYK
LGQKGQFFSVYIFWGWIINGFFHSAIVFIGTILIYRYGFALNMHGELADH
WSWGVTVYTTSVIIVLGKAALVTNQWTKFTLIAIPGSLLFWLIFFPIYAS
IFPHANISREYYGVVKHTYGSGVFWLTLIVLPIFALVRDFLWKYYKRMYE
PETYHVIQEMQKYNIS

3D structure

• PDB: 7pem Substrate Transport and Specificity in a Phospholipid Flippase
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.1: P-type phospholipid transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	2Y5	A	W1028 G1096 F1103 W1223 K1224 H1236	W847 G915 F922 W1042 K1043 H1055
BS02	MG	A	X560 T562 D954	X379 T381 D773
BS03	ATP	A	D654 E655 F698 K703 R704 M705 K721 A723 L757 R838 R928	D473 E474 F517 K522 R523 M524 K540 A542 L576 R657 R747
BS04	Q3G	A	T253 S509 F1048 L1051 F1052 W1056 F1171 F1175	T72 S328 F867 L870 F871 W875 F990 F994

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0000287 magnesium ion binding
• GO:0005215 transporter activity
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008289 lipid binding
• GO:0016887 ATP hydrolysis activity
• GO:0046872 metal ion binding
• GO:0070273 phosphatidylinositol-4-phosphate binding
• GO:0090555 phosphatidylethanolamine flippase activity
• GO:0090556 phosphatidylserine floppase activity
• GO:0140326 ATPase-coupled intramembrane lipid transporter activity
• GO:0140345 phosphatidylcholine flippase activity
• GO:0140346 phosphatidylserine flippase activity

Biological Process

• GO:0006892 post-Golgi vesicle-mediated transport
• GO:0006897 endocytosis
• GO:0015914 phospholipid transport
• GO:0032456 endocytic recycling
• GO:0045332 phospholipid translocation
• GO:0140331 aminophospholipid translocation

Cellular Component

• GO:0005768 endosome
• GO:0005783 endoplasmic reticulum
• GO:0005794 Golgi apparatus
• GO:0005802 trans-Golgi network
• GO:0005886 plasma membrane
• GO:0010008 endosome membrane
• GO:0016020 membrane
• GO:1990530 Cdc50p-Drs2p complex
• GO:1990531 phospholipid-translocating ATPase complex

External links

• PDB: RCSB:7pem, PDBe:7pem, PDBj:7pem
• PDBsum: 7pem
• UniProt: P39524|ATC3_YEAST Phospholipid-transporting ATPase DRS2 (Gene Name=DRS2)