Structure of PDB 7p5z Chain A

Receptor sequence
>7p5zA (length=623) Species: 559292 (Saccharomyces cerevisiae S288C) [Search protein sequence]
LESLSNVKANSYSEWITQPNVSRTIARELKSFLLEYTDETGRSVYGARIR
TLGEMNSESLEVNYRHLAESKAILALFLAKCPEEMLKIFDLVAMEATELH
YPDYARIHSEIHVRISDFPTIYSLRELRESNLSSLVRVTGVVTRRTGVFP
QLKYVKFNCLKCGSILGPFFQDSNEEIRISFCTNCKSKGPFRVNGEKTVY
RNYQRVTLQEAPGTVPPGRLPRHREVILLADLVDVSKPGEEVEVTGIYKN
NYDGNLNAKNGFPVFATIIEANSIKRRVFSWTEEEEREFRKISRDRGIID
KIISSMAPSIYGHRDIKTAVACSLFGGVPKNVNGKHSIRGDINVLLLGDP
GTAKSQILKYVEKTAHRAVFATGQGASAVGLTASVRKDPITKEWTLEGGA
LVLADKGVCLIDEFDKMNDQDRTSIHEAMEQQSISISKAGIVTTLQARCS
IIAAANPNGGRYNSTLPLAQNVSLTEPILSRFDILCVVRDLVDEEADERL
ATFVVDSHVRSHPESPIPQELLMKYIHYARTKIYPKLHQMDMDKVSRVYA
DLRRESISTGSFPITVRHLESILRIAESFAKMRLSEFVSSYDLDRAIKVV
VDSFVDAQKVSVRRQLRRSFAIY
3D structure
PDB7p5z Structural mechanism for the selective phosphorylation of DNA-loaded MCM double hexamers by the Dbf4-dependent kinase.
ChainA
Resolution3.3 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.6.4.12: DNA helicase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna A P372 K587 P190 K392
BS02 ATP A G546 T547 A548 K549 S550 Q551 L695 G351 T352 A353 K354 S355 Q356 L500
BS03 ZN A C341 C344 C367 C159 C162 C185
BS04 ADP A V807 R808 E811 V566 R567 E570
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003682 chromatin binding
GO:0003688 DNA replication origin binding
GO:0003697 single-stranded DNA binding
GO:0004386 helicase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016787 hydrolase activity
GO:0016887 ATP hydrolysis activity
GO:0017116 single-stranded DNA helicase activity
GO:0043138 3'-5' DNA helicase activity
GO:0046872 metal ion binding
Biological Process
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006267 pre-replicative complex assembly involved in nuclear cell cycle DNA replication
GO:0006268 DNA unwinding involved in DNA replication
GO:0006270 DNA replication initiation
GO:0006271 DNA strand elongation involved in DNA replication
GO:0006279 premeiotic DNA replication
GO:0006974 DNA damage response
GO:0032508 DNA duplex unwinding
GO:1902975 mitotic DNA replication initiation
GO:1905775 negative regulation of DNA helicase activity
Cellular Component
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005656 nuclear pre-replicative complex
GO:0005737 cytoplasm
GO:0031261 DNA replication preinitiation complex
GO:0031298 replication fork protection complex
GO:0042555 MCM complex
GO:0043596 nuclear replication fork
GO:0071162 CMG complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:7p5z, PDBe:7p5z, PDBj:7p5z
PDBsum7p5z
PubMed34963704
UniProtP29469|MCM2_YEAST DNA replication licensing factor MCM2 (Gene Name=MCM2)

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