Structure of PDB 7ow8 Chain A

Receptor sequence

>7ow8A (length=577) Species: 224308 (Bacillus subtilis subsp. subtilis str. 168)

SKLKPFFALVRRTNPSYGKLAFALALSVVTTLVSLLIPLLTKQLVDGFSM
SNLSGTQIGLIALVFFVQAGLSAYATYALNYNGQKIISGLRELLWKKLIK
LPVSYFDTNASGETVSRVTNDTMVVKELITTHISGFITGIISVIGSLTIL
FIMNWKLTLLVLVVVPLAALILVPIGRKMFSISRETQDETARFTGLLNQI
LPEIRLVKASNAEDVEYGRGKMGISSLFKLGVREAKVQSLVGPLISLVLM
AALVAVIGYGGMQVSSGELTAGALVAFILYLFQIIMPMGQITTFFTQLQK
SIGATERMIEILAEEEEDTVTGKQIENAHLPIQLDRVSFGYKPDQLILKE
VSAVIEAGKVTAIVGPSGGGKTTLFKLLERFYSPTAGTIRLGDEPVDTYS
LESWREHIGYVSQESPLMSGTIRENICYGLERDVTDAEIEKAAEMAYALN
FIKELPNQFDTEVGERGIMLSGGQRQRIAIARALLRNPSILMLDAATSSL
DSQSEKSVQQALEVLMEGRTTIVIAHRLSTVVDADQLLFVEKGEITGRGT
HHELMASHGLYRDFAEQQLKMNADLEN

3D structure

• PDB: 7ow8 Substrate-bound and substrate-free outward-facing structures of a multidrug ABC exporter.
• Chain: A
• Resolution: 3.5 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	D116 Y350 S376 G377 G379 K380 T381 T382 Q422 H535	D107 Y341 S367 G368 G370 K371 T372 T373 Q413 H526
BS02	MG	A	T381 Q422	T372 Q413
BS03	ATP	A	I477 S480 G482	I468 S471 G473

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0016887 ATP hydrolysis activity
• GO:0034040 ATPase-coupled lipid transmembrane transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006869 lipid transport
• GO:0046677 response to antibiotic
• GO:0055085 transmembrane transport

Cellular Component

• GO:0005886 plasma membrane
• GO:0016020 membrane

External links

• PDB: RCSB:7ow8, PDBe:7ow8, PDBj:7ow8
• PDBsum: 7ow8
• PubMed: 35080979
• UniProt: O06967|BMRA_BACSU Multidrug resistance ABC transporter ATP-binding/permease protein BmrA (Gene Name=bmrA)