Structure of PDB 7osz Chain A

Receptor sequence

>7oszA (length=486) Species: 9606 (Homo sapiens)

LEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFD
AEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEP
IAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTR
EFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEK
FAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEK
QFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGEDKEA
LIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPR
DMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKT
HMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLESMGA
KSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

3D structure

• PDB: 7osz Towards Novel 3-Aminopyrazinamide-Based Prolyl-tRNA Synthetase Inhibitors: In Silico Modelling, Thermal Shift Assay and Structural Studies.
• Chain: A
• Resolution: 2.46 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.15: proline--tRNA ligase.
  6.1.1.17: glutamate--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PRO	A	T1121 E1123 W1169 E1171 H1173 F1216 H1242 W1273 G1274	T106 E108 W154 E156 H158 F201 H227 W258 G259
BS02	0X7	A	F1161 L1162 T1164 F1167 G1274 T1276 R1278	F146 L147 T149 F152 G259 T261 R263
BS03	ZN	A	C1448 C1495 C1497	C426 C469 C471

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004827 proline-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006433 prolyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:7osz, PDBe:7osz, PDBj:7osz
• PDBsum: 7osz
• PubMed: 34360555
• UniProt: P07814|SYEP_HUMAN Bifunctional glutamate/proline--tRNA ligase (Gene Name=EPRS1)