Structure of PDB 7orz Chain A

Receptor sequence

>7orzA (length=337) Species: 208964 (Pseudomonas aeruginosa PAO1)

LELQEHCSLKPYNTFGIDVRARLLAHARDEADVREALALARERGLPLLVI
GGGSNLLLTRDVEALVLRMASQGRRIVSDAADSVLVEAEAGEAWDPFVQW
SLERGLAGLENLSLIPGTVGAAPMQNIGAYGVELKDVFDSLTALDRQDGT
LREFDRQACRFGYRDSLFKQEPDRWLILRVRLRLTRRERLHLDYGPVRQR
LEEEGIASPTARDVSRVICAIRREKLPDPAVLGNAGSFFKNPLVDATQAE
RLRQAFPDLVGYPQADGRLKLAAGWLIDKGGWKGFRDGPVGVHAQQALVL
VNHGGATGAQVRALAERIQEDVRRRFGVELEPEPNLY

3D structure

• PDB: 7orz Discovery of Novel Inhibitors of Uridine Diphosphate- N -Acetylenolpyruvylglucosamine Reductase (MurB) from Pseudomonas aeruginosa , an Opportunistic Infectious Agent Causing Death in Cystic Fibrosis Patients.
• Chain: A
• Resolution: 1.85 Å

Enzymatic activity

• Enzyme Commision number: 1.3.1.98: UDP-N-acetylmuramate dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	0IM	A	A131 Y132 L228 G238 S239 V301	A129 Y130 L226 G236 S237 V299
BS02	FAD	A	V51 G53 G54 G55 S56 N57 L58 I117 P118 G119 T120 A123 M126 I129 G130 R166 I179 R224 P231 N236 A237 G238 N337 Y339	V49 G51 G52 G53 S54 N55 L56 I115 P116 G117 T118 A121 M124 I127 G128 R164 I177 R222 P229 N234 A235 G236 N335 Y337

Gene Ontology

Molecular Function

• GO:0008762 UDP-N-acetylmuramate dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0071949 FAD binding

Biological Process

• GO:0008360 regulation of cell shape
• GO:0009252 peptidoglycan biosynthetic process
• GO:0051301 cell division
• GO:0071555 cell wall organization

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7orz, PDBe:7orz, PDBj:7orz
• PDBsum: 7orz
• PubMed: 35080396
• UniProt: Q9HZM7|MURB_PSEAE UDP-N-acetylenolpyruvoylglucosamine reductase (Gene Name=murB)