Structure of PDB 7oro Chain A

Receptor sequence

>7oroA (length=2008)

MDYQEYQQFLARINTARDACVAKDIDVDLLMARKDYFGRELCKSLNIEYR
NDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSNE
SSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELYP
TIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCPE
FWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKKD
YSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLHD
QKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGKM
MDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQFM
INNDLIDKSEKLKLFKNFCGIGKHVSKPKILDFDDANMYLASLTMMEQSK
KILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDFS
TLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSAKATVVYSIIVLHKE
EENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLFLTTCLL
FKHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLAISSNVK
DYIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYLSDYDIT
QKGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHHVMVDLA
KTILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADTSRHNHL
RNRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKEYEMLRNAMPNYTD
YISTKVFDRLYELLDKKVLTDKPVIEQIMDMMIDHKKFYFTFFNKGQKTS
KDREIFVGEYEAKMCMYAVERIAKERCKLNPDEMISEPGDGKLKVLEQKS
EQEIRFLVETTRQKNREIDEAIEIEKLSLGKAKGLKMEINADMSKWSAQD
VFYKYFWLIALDPILYPQEKERILYFMCNYMDKELILPDELLFNLLDQKV
AYQNDIIATMTNQLNSNTVLIKRNWLQGNFNYTSSYVHSCAMSVYKEILK
EAITLLDGSILVNSLVHSDDNQTSITIVQDKMENDKIIDFAMKEFERACL
TFGCQANMKKTYVTNCIKEFVSLFNLYGEPFSIYGRFLLTSVGDCAYIGP
YEDLASRISSAQTAIKHGCPPSLAWVSIAISHWMTSLTYNMLPGQSNDPI
DYFPAENRKDIPIELNGVLDAPLSMISTVGLESGNLYFLIKLLSKYTPVM
QKRESVVNQIAEVKNWKVEDLTDNEIFRLKILRYLVLDAEMDPSDIMGET
SDMRGRSILTPRKFTTAGSLRKLYSFSKYQDRLSSPGGMVELFTYLLEKP
ELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQNPAQLFIEQILFSHKPV
IDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLELTNDDIQVIYSYIILND
PMMITIANTHILSIYGSPQRRMGMSCSTMPEFRNLKLIHHSPALVLRAYS
KNNPDIQGADPTEMARDLVHLKEFVENTNLEEKMKVRIAMNEAEKGQRDI
VFELKEMTRFYQVCYEYVKSTEHKIKVFILPAKSYTTTDFCSLMQGNLIK
DKEWYTVHYLKQILSGGHKAIMQHNATSEQNIAFECFKLITHFADSFIDS
LSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLFRTGDLRQA
DLDKYDAMKILIRSLDYLNNDIFSLSRIKVGLDEFATIKKAHFSKMVSFE
GPPIKTGLLDLTELMKSQDLLNLNYDNIRNSNLISFSKLICCEGSDNIND
GLEFLSDDPMNFTEGEAIHSTPIFNIYYSKRGERHMTYRNAIKLLIERET
KIFEEAFTFSENGFISPENLGCLEAVVSLIKLLKTNEWSTVIDKCIHICL
IKNGMDHMYHSFDVPKCFINWVMFREFINSLPGTDIPPWNVMTENFKKKC
IALINSKF

3D structure

• PDB: 7oro Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Q291 R292 K302 H306 C419 G420 G422 K423 K439 P440 R592 A593 R595 R600 T642 K643 Y677 E758 H760 H761 V764 K768 L1113 Q1116 V1118 Y1120 D1123	Q291 R292 K302 H306 C419 G420 G422 K423 K427 P428 R575 A576 R578 R583 T625 K626 Y660 E741 H743 H744 V747 K751 L1045 Q1048 V1050 Y1052 D1055
BS02	rna	A	H312 N313 N318 K368 R372 Q377 I378 K381 K382 L383 W395 E396 Q397 L471 K472 N517 S520 V521 Y524 R526 H527 R531 M534 C535 A536 N537 N538 N1308 I1511 L1512 H1515 K1516	H312 N313 N318 K368 R372 Q377 I378 K381 K382 L383 W395 E396 Q397 L459 K460 N505 S508 V509 Y512 R514 H515 R519 M522 C523 A524 N525 N526 N1240 I1443 L1444 H1447 K1448
BS03	rna	A	K841 S842 S908 F948 I960 F961 V962 R976 K979 P993 G994 G1146 Y1150 Y1269 A1273 S1425 K1705 I1706	K824 S825 S853 F893 I905 F906 V907 R921 K924 P938 G939 G1078 Y1082 Y1201 A1205 S1357 K1624 I1625
BS04	rna	A	Y750 K754 S840 D1187 S1240 R1254 F1255 A1264 V1472 R1493 S1497	Y733 K737 S823 D1119 S1172 R1186 F1187 A1196 V1404 R1425 S1429
BS05	ZN	A	C2064 H2169 H2182	C1842 H1947 H1960

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:7oro, PDBe:7oro, PDBj:7oro
• PDBsum: 7oro
• PubMed: 35173159
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)