Structure of PDB 7orm Chain A

Receptor sequence

>7ormA (length=2145) Species: 11577 (La Crosse virus)

MDYQEYQQFLARINTARDACVAKDIDVDLLMARKDYFGRELCKSLNIEYR
NDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSNE
SSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELYP
TIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCPE
FWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKKD
YSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLHD
QKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGKM
MDIGDKAIEYEEFCMSLKSKARSSWKQIMNKKLEPKQINNALVLWEQQFM
INNDLIDKSEKLKLFKNFCGIGKHKQEVSKPKILDFDDANMYLASLTMME
QSKKILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCIS
DFSTLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSADIKTKKATVVY
SIIVLHKEEENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPG
LFLTTCLLFKHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNS
LAISSNVKDYIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDI
YLSDYDITQKGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEK
HHVMVDLAKTILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLA
DTSRHNHLRNRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKENYEML
RNAMPNYTDYISTKVFDRLYELLDKKVLTDKPVIEQIMDMMIDHKKFYFT
FFNKGQKTSKDREIFVGEYEAKMCMYAVERIAKERCKLNPDEMISEPGDG
KLKVLEQKSEQEIRFLVETTRQKNREIDEAIEIEKLSLGKAKGLKMEINA
DMSKWSAQDVFYKYFWLIALDPILYPQEKERILYFMCNYMDKELILPDEL
LFNLLDQKVAYQNDIIATMTNQLNSNTVLIKRNWLQGNFNYTSSYVHSCA
MSVYKEILKEAITLLDGSILVNSLVHSDDNQTSITIVQDKMENDKIIDFA
MKEFERACLTFGCQANMKKTYVTNCIKEFVSLFNLYGEPFSIYGRFLLTS
VGDCAYIGPYEDLASRISSAQTAIKHGCPPSLAWVSIAISHWMTSLTYNM
LPGQSNDPIDYFPAENRKDIPIELNGVLDAPLSMISTVGLESGNLYFLIK
LLSKYTPVMQKRESVVNQIAEVKNWKVEDLTDNEIFRLKILRYLVLDAEM
DPSDIMGETSDMRGRSILTPRKFTTAGSLRKLYSFSKYQDRLSSPGGMVE
LFTYLLEKPELLVTKGEDMKDYMESVIFRYNSKRFKESLSIQNPAQLFIE
QILFSHKPVIDFSGIRDKYINLGKVTFTEAYRLLMRDLSSLELTNDDIQV
IYSYIILNDPMMITIANTHILSIYGSPQRRMGMSCSTMPEFRNLKLIHHS
PALVLRAYSKNNPDIQGADPTEMARDLVHLKEFVENTNLEEKMKVRIAMN
EAEKGQRDIVFELKEMTRFYQVCYEYVKSTEHKIKVFILPAKSYTTTDFC
SLMQGNLIKDKEWYTVHYLKQILSGGHKAIMQHNATSEQNIAFECFKLIT
HFADSFIDSLSRSAFLQLIIDEFSYKDVKVSKLYDIIKNGYNRTDFIPLL
FRTGDLRQADLDKYDAMKSHERVTLTITGYNRSITIIGEDNKLTYAELCL
TRKTPENITISGRKLLGSRHGLKFENMSKIQNYYITYRKKDRHQFVYQIH
SHESITRRNEEHNEITPVCVVNVAEVDGDQRILIRSLDYLNNDIFSLSRI
KVGLDEFATIKKAHFSKMVSFEGPPIKTGLLDLTELMKSQDLLNLNYDNI
RNSNLISFSKLICCEGSDNINDGLEFLSDDPMNFTEGEAIHSTPIFNIYY
SKRGERHMTYRNAIKLLIERETKIFEEAFTFSENGFISPENLGCLEAVVS
LIKLLKTNEWSTVIDKCIHICLIKNGMDHMYHSFDVPKCFMGNPITRDIN
WVMFREFINSLPGTDIPPWNVMTENFKKKCIALINSKFSEFTKLM

3D structure

• PDB: 7orm Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Q291 R292 K302 H306 G420 I421 G422 Q426 P440 R592 A593 R595 R600 T642 K643 Y677 E758 H760 H761 V764 K768 L1113 Q1116 V1118 Y1120 D1123	Q291 R292 K302 H306 G420 I421 G422 Q426 P431 R583 A584 R586 R591 T633 K634 Y668 E749 H751 H752 V755 K759 L1054 Q1057 V1059 Y1061 D1064
BS02	rna	A	S842 F948 K950 I960 F961 V962 R976 K979 P993 G994 Q1145 G1146 Y1150 Y1269 S1277 S1425 R1493 K1705 I1706 K1707	S833 F902 K904 I914 F915 V916 R930 K933 P947 G948 Q1086 G1087 Y1091 Y1210 S1218 S1366 R1434 K1633 I1634 K1635
BS03	rna	A	R33 V134 F162 K165 H184 Y750 N823 R824 S840 H1185 D1187 V1239 S1240 R1254 F1255 D1262 V1472 K1474 S1497 S1622 Q1693 Y1696	R33 V134 F162 K165 H184 Y741 N814 R815 S831 H1126 D1128 V1180 S1181 R1195 F1196 D1203 V1413 K1415 S1438 S1550 Q1621 Y1624
BS04	ZN	A	C2064 H2169 D2178 H2182	C1964 H2069 D2078 H2082

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:7orm, PDBe:7orm, PDBj:7orm
• PDBsum: 7orm
• PubMed: 35173159
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)