Structure of PDB 7ork Chain A

Receptor sequence

>7orkA (length=2182) Species: 11577 (La Crosse virus)

MDYQEYQQFLARINTARDACVAKDIDVDLLMARKDYFGRELCKSLNIEYR
NDVPFIDIILDIRPEVDPLTIDAPHITPDNYLYINNVLYIIDYKVSVSNE
SSVITYDKYYELTRDISDRLSIPIEIVIIRIDPVSRDLHINSDRFKELYP
TIVVDINFNQFFDLKQLLYEKFGDDEEFLLKVAHGDFTLTAPWCKTGCPE
FWKHPIYKEFKMSMPVPERRLFEESVKFNAYESERWNTNLVKIREYTKKD
YSEHISKSAKNIFLASGFYKQPNKNEISEGWTLMVERVQDQREISKSLHD
QKPSIHFIWGAHNPGNSNNATFKLILLSKSLQSIKGISTYTEAFKSLGKM
MDIGDKAIEYEEFCMSLKSKALEPKQINNALVLWEQQFMINNDLIDKSEK
LKLFKNFCGIGKHKQFKNKMLEDLEVSKPKILDFDDANMYLASLTMMEQS
KKILSKSNGLKPDNFILNEFGSRIKDANKETYDNMHKIFETGYWQCISDF
STLMKNILSVSQYNRHNTFRIAMCANNNVFAIVFPSADIKTKKATVVYSI
IVLHKEEENIFNPGCLHGTFKCMNGYISISRAIRLDKERCQRIVSSPGLF
LTTCLLFKHDNPTLVMSDIMNFSIYTSLSITKSVLSLTEPARYMIMNSLA
ISSNVKDYIAEKFSPYTKTLFSVYMTRLIKNACFDAYDQRQRVQLRDIYL
SDYDITQKGIKDNRELTSIWFPGSVTLKEYLTQIYLPFYFNAKGLHEKHH
VMVDLAKTILEIECEQRENIKEIWSTNCTKQTVNLKILIHSLCKNLLADT
SRHNHLRNRIENRNNFRRSITTISTFTSSKSCLKIGDFRKEKELRKMRLA
NPMFVTDEQVCLEVGHCNYEMLRNAMPNYTDYISTKVFDRLYELLDKKVL
TDKPVIEQIMDMMIDHKKFYFTFFNKGQKTSKDREIFVGEYEAKMCMYAV
ERIAKERCKLNPDEMISEPGDGKLKVLEQKSEQEIRFLVETTRQKNREID
EAIEALATLGKIEKLSLGKAKGLKMEINADMSKWSAQDVFYKYFWLIALD
PILYPQEKERILYFMCNYMDKELILPDELLFNLLDQKVAYQNDIIATMTN
QLNSNTVLIKRNWLQGNFNYTSSYVHSCAMSVYKEILKEAITLLDGSILV
NSLVHSDDNQTSITIVQDKMENDKIIDFAMKEFERACLTFGCQANMKKTY
VTNCIKEFVSLFNLYGEPFSIYGRFLLTSVGDCAYIGPYEDLASRISSAQ
TAIKHGCPPSLAWVSIAISHWMTSLTYNMLPGQSNDPIDYFPAENRKDIP
IELNGVLDAPLSMISTVGLESGNLYFLIKLLSKYTPVMQKRESVVNQIAE
VKNWKVEDLTDNEIFRLKILRYLVLDAEMDPSDIMGETSDMRGRSILTPR
KFTTAGSLRKLYSFSKYQDRLSSPGGMVELFTYLLEKPELLVTKGEDMKD
YMESVIFRYNSKRFKESLSIQNPAQLFIEQILFSHKPVIDFSGIRDKKEP
DILGKVTFTEAYRLLMRDLSSLELTNDDIQVIYSYIILNDPMMITIANTH
ILSIYGSPQRRMGMSCSTMPEFRNLKLIHHSPALVLRAYSKNNPDIQGAD
PTEMARDLVHLKEFVENTNLEEKMKVRIAMNEAEKGQRDIVFELKEMTRF
YQVCYEYVKSTEHKIKVFILPAKSYTTTDFCSLMQGNLIKDKEWYTVHYL
KQILSGGHKAIMQHNATSEQNIAFECFKLITHFADSFIDSLSRSAFLQLI
IDEFSYKDVKVSKLYDIIKNGYNRTDFIPLLFRTGDLRQADLDKYDAMKS
HERVTWNDWQTSRHLDMGSINLTITGYNRSITIIGEDNKLTYAELCLTRK
TPENITISGRKLLGSRHGLKFENMSKIQTYPGNYYITYRKKDRHQFVYQI
HSHESITRRNEEHMAIIYNEITPVCVVNVAEVDGDQRILIRSLDYLNNDI
FSLSRIKVGLDEFATIKKAHFSKMVSFEGPPIKTGLLDLTELMKSQDLLN
LNYDNIRNSNLISFSKLICCEGSDNINDGLEFLSDDPMNFTEGEAIHSTP
IFNIYYSKRGERHMTYRNAIKLLIERETKIFEEAFTFSENGFISPENLGC
LEAVVSLIKLLKTNEWSTVIDKCIHICLIKNGMDHMYHSFDVPINWVMFR
EFINSLPGTDIPPWNVMTENFKKKCIALINSK

3D structure

• PDB: 7ork Structural snapshots of La Crosse virus polymerase reveal the mechanisms underlying Peribunyaviridae replication and transcription.
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.48: RNA-directed RNA polymerase.
  3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	Q291 R292 K302 H306 C419 G420 G422 H424 Q426 K430 L435 K439 P440 A548 R592 A593 R595 R600 T642 K643 Y677 K679 E758 H760 H761 V764 K768 H890 Q1116 V1118 Y1120 D1123	Q291 R292 K302 H306 C408 G409 G411 H413 Q415 K419 L424 K428 P429 A537 R581 A582 R584 R589 T631 K632 Y666 K668 E747 H749 H750 V753 K757 H866 Q1086 V1088 Y1090 D1093
BS02	rna	A	H527 N528 D549 K551 T552 K553 K598 K841 S842 F948 K950 I960 F961 K968 R976 K979 M989 I990 S991 G1146 Y1150 F1432 T1434 I1500 N1502 P1503 Q1505 E1509 K1516 D1542	H516 N517 D538 K540 T541 K542 K587 K830 S831 F924 K926 I936 F937 K944 R952 K955 M965 I966 S967 G1116 Y1120 F1402 T1404 I1470 N1472 P1473 Q1475 E1479 K1486 D1501
BS03	rna	A	Y714 W1847 W1850 Q1851 R1854 H1908 G1909 K2012 A2013 H2014 K2017	Y703 W1806 W1809 Q1810 R1813 H1867 G1868 K1968 A1969 H1970 K1973
BS04	MG	A	D1060 D1187 D1188	D1030 D1157 D1158
BS05	MG	A	D1060 M1061 D1187	D1030 M1031 D1157
BS06	ZN	A	H2169 D2178 H2182	H2125 D2134 H2138
BS07	ATP	A	R958 D1060 M1061 W1064 S1065 Q1145 G1146 D1187	R934 D1030 M1031 W1034 S1035 Q1115 G1116 D1157

Gene Ontology

Molecular Function

• GO:0003968 RNA-dependent RNA polymerase activity

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0019079 viral genome replication
• GO:0039694 viral RNA genome replication

External links

• PDB: RCSB:7ork, PDBe:7ork, PDBj:7ork
• PDBsum: 7ork
• PubMed: 35173159
• UniProt: A5HC98|L_BUNLC RNA-directed RNA polymerase L (Gene Name=L)