Structure of PDB 7ok0 Chain A

Receptor sequence

>7ok0A (length=880) Species: 330779 (Sulfolobus acidocaldarius DSM 639)

MSEKIIRGVKFGVLSPNEIRQMSVTAIITSEVYDEDGTPIEGGVMDPKLG
VIEPGQKCPVCGNTLAGCPGHFGHIELIKPVIHIGYVKHIYDFLRSTCWR
CGRIKIKEQDLERYKRIYNAIKLRWPSAARRLVEYIKKISIKNLECPHCG
EKQFKIKLEKPYNFNEERNGSIVKLSPSEIRDRLERIPDSDVELLGYDPK
SSRPEWMILTVLPVPPITIRPSITIESGIRAEDDLTHKLVDIIRLNERLK
ESIEAGAPQLIIEDLWDLLQYHVATYFDNEIPGLPPAKHRSGRPLRTLAQ
RLKGKEGRFRGNLSGKRVDFSARTVISPDPNLSIDEVGIPYTIARMLTVP
ERVTNINIERIRQYIINGPDKWPGANYVIKPDGRRIDLRYVKDRKELASS
ITAGYVVERHLVDGDVVLFNRQPSLHRISMMAHKVRVLPGRTFRLNLLDC
PPYNADFDGDEMNLHVPQSEEAIAEARELMLVHKNIITPRYGGPIIGGGQ
DYISGAYLLSVKTTLLTVEEVATILGVTDFVGELGEPAILAPKPYYTGKQ
VISLFLPKDFNFHGPANISKGPRACKDEICPHDSFIVIKNGLLLEGVFDK
KAIGNQQPESMLHWSIREYGTEYGKWLMDNVFKMFIRFLEMRGFTMTLED
ITIPDEAQNEITTKIKEGYSQVDEYIRKFNEGQLEPIPGRTIEESLESYI
LDTLDKLRKVAGEIATKYLDPFNNVYIMAITGARGSELNITQMTALLGQQ
SVRGERIRRGYRERTLSLFKYGDIAPEARGFVKNSFMRGLSPYEMFFHAA
GGREGLVDTAVKTSQSGYMQRRLINALSDLRIEYDGTVRSLYGDIVQVVY
GDDAVHPMYSAHSKSVNVNRVIERVIGWKR

3D structure

• PDB: 7ok0 Structural basis of RNA polymerase inhibition by viral and host factors.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	A	D456 D458 D460	D456 D458 D460
BS02	ZN	A	C58 H71	C58 H71
BS03	ZN	A	C98 C146 C149	C98 C146 C149
BS04	ZN	A	R573 H582 S584	R573 H582 S584

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0003677 DNA binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005737 cytoplasm

External links

• PDB: RCSB:7ok0, PDBe:7ok0, PDBj:7ok0
• PDBsum: 7ok0
• PubMed: 34535646
• UniProt: P11512|RPO1N_SULAC DNA-directed RNA polymerase subunit Rpo1N (Gene Name=rpo1N)