Structure of PDB 7obb Chain A

Receptor sequence

>7obbA (length=1510) Species: 9606 (Homo sapiens)

KNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLA
LGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSC
LNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSA
SEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKR
CPHCKTGRSVVRKEHNSKLTITFGYLTPTSAREHLSALWKNEGFFLNYLF
SGMRFNPSVFFLDFLVVPPSRYRPVSRLGDQMFTNGQTVNLQAVMKDVVL
IRKLLALMAQFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEMDKLMMDK
YPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEIGIPMVFA
TKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTALSAVDMT
QREAVAKQLLTKIVCRHVKNGDILLLNRQPTLHRPSIQAHRARILPEEKV
LRLHYANCKAYNADFDGDEMNAHFPQSELGRAEAYVLACTDQQYLVPKDG
QPLAGLIQDHMVSGASMTTRGCFFTREHYMELVYRGLTDKVGRVKLLSPS
ILKPFPLWTGKQVVSTLLINIIPEDHIPLNLSGKAKITGKAWVKETPRSV
PGFNPDSMCESQVIIREGELLCGVLDKAHYGSSAYGLVHCCYEIYGGETS
GKVLTCLARLFTAYLQLYRGFTLGVEDILVKPKADVKRQRIIEESTHCGP
QAVRAALNLPEAASYDEVRGKWQDAHLGKDQRDFNMIDLKFKEEVNHYSN
EINKACMPFGLHRQFPENSLQMMVQSGAKGSTVNTMQISCLLGQIELEGR
RPPLMASGKSLPCFEPYEFTPRAGGFVTGRFLTGIKPPEFFFHCMAGREG
LVDTAVKGYLQRCIIKHLEGLVVQYDLTVRDSDGSVVQFLYGEDGLDIPK
TQFLQPKQFPFLASNYEVIMKSQHLHEVLSRADPKKALHHFRAIKKWQSK
HPNTLLRRGAFLSYSQKIQEAVKALKLESENRNGRSPGTQEMLRMWYELD
EESRRKYQKKAAACPDPSLSVWRPDIYFASVSETFETKVDDYSQEWAAQT
EKSYEKSELSLDRLRTLLQLKWQRSLCEPGEAVGLLAAQSIGEPSTQMTL
NMNVTLGIPRLREILMVASANIKTPMMSVPVLNTKKALKRVKSLKKQLTR
VCLGEVLQKIDVQESFCMEEKQNKFQVYQLRFQFLPHAYYQQEKCLRPED
ILRFMETRFFKLLMESIKKKNNKRNVNTRRATQRDEEGHIVRVQAVREIH
PFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSLAHGAVIYATKGI
TRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDLRRLYSNDIHAIA
NTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADYMCFEGVYKPLNR
FGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPSACLVVGKVVRGG
TGLFELKQPL

3D structure

• PDB: 7obb Cryo-EM structures of human RNA polymerase I.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	E405 R418 R429 Y987 E1675	E342 R355 R366 Y909 E1466
BS02	dna	A	K197 V1254 F1660	K193 V1167 F1451
BS03	ZN	A	C67 H77	C63 H73
BS04	ZN	A	C104 C205	C100 C201

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003682 chromatin binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding
• GO:0071667 DNA/RNA hybrid binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006360 transcription by RNA polymerase I
• GO:1904750 negative regulation of protein localization to nucleolus

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005736 RNA polymerase I complex

External links

• PDB: RCSB:7obb, PDBe:7obb, PDBj:7obb
• PDBsum: 7obb
• PubMed: 34887565
• UniProt: O95602|RPA1_HUMAN DNA-directed RNA polymerase I subunit RPA1 (Gene Name=POLR1A)