Structure of PDB 7oba Chain A

Receptor sequence

>7obaA (length=1522) Species: 9606 (Homo sapiens)

KNMPWRRLQGISFGMYSAEELKKLSVKSITNPRYLDSLGNPSANGLYDLA
LGPADSKEVCSTCVQDFSNCSGHLGHIELPLTVYNPLLFDKLYLLLRGSC
LNCHMLTCPRAVIHLLLCQLRVLEVGALQAVYELERILNRFLEENPDPSA
SEIREELEQYTTEIVQNNLLGSQGAHVKNVCESKSKLIALFWKAHMNAKR
CPHCKTGRSVVRKEHNSKLTITFPAGYLTPTSAREHLSALWKNEGFFLNY
LFSGMDDDGMESRFNPSVFFLDFLVVPPSRYRPVNGQTVNLQAVMKDVVL
IRKLLALMAQEQKLPEEFLSTLPGQSLIDKLYNIWIRLQSHVNIVFDSEM
DKLMMDKYPGIRQILEKKEGLFRKHMMGKRVDYAARSVICPDMYINTNEI
GIPMVFATKLTYPQPVTPWNVQELRQAVINGPNVHPGASMVINEDGSRTA
LSAVDMTQREAVAKQLLTPATGAPKPQGTKIVCRHVKNGDILLLNRQPTL
HRPSIQAHRARILPEEKVLRLHYANCKAYNADFDGDEMNAHFPQSELGRA
EAYVLACTDQQYLVPKDGQPLAGLIQDHMVSGASMTTRGCFFTREHYMEL
VYRGLTDKVGRVKLLSPSILKPFPLWTGKQVVSTLLINIIPEDHIPLNLS
GKAKITGKAWVKETPRSVPGFNPDSMCESQVIIREGELLCGVLDKAHYGS
SAYGLVHCCYEIYGGETSGKVLTCLARLFTAYLQLYRGFTLGVEDILVKP
KADVKRQRIIEESTHCGPQAVRAALNLPEAASYDEVRGKWQDAHLGKDQR
DFNMIDLKFKEEVNHYSNEINKACMPFGLHRQFPENSLQMMVQSGAKGST
VNTMQISCLLGQIELEGRRPPLMASGKSLPCFEPYEFTPRAGGFVTGRFL
TGIKPPEFFFHCMAGREGLVDTAVKSGYLQRCIIKHLEGLVVQYDLTVRD
SDGSVVQFLYGEDGLDIPKTQFLQPKQFPFLASNYEVIMKSQHLHEVLSR
ADPKKALHHFRAIKKWQSKHPNTLLRRGAFLSYSQKIQEAVKALKLESEN
RNGRSPGTQEMLRMWYELDEESRRKYQKKAAACPDPSLSVWRPDIYFASV
SETFETKVDDYSQEWAAQTEKSYEKSELSLDRLRTLLQLKWQRSLCEPGE
AVGLLAAQSIGEPSTQMTLNMNVTLGIPRLREILMVASANIKTPMMSVPV
LNTKKALKRVKSLKKQLTRVCLGEVLQKIDVQESFCMEEKQNKFQVYQLR
FQFLPHAYYQQEKCLRPEDILRFMETRFFKLLMESIKKKNNKRNVNTRRA
TQRRVQAVREIHPFIDDYQYDTEESLWCQVTVKLPLMKINFDMSSLVVSL
AHGAVIYATKGITRCLLNETTNNKNEKELVLNTEGINLPELFKYAEVLDL
RRLYSNDIHAIANTYGIEAALRVIEKEIKDVFAVYGIAVDPRHLSLVADY
MCFEGVYKPLNRFGIRSNSSPLQQMTFETSFQFLKQATMLGSHDELRSPS
ACLVVGKVVRGGTGLFELKQPL

3D structure

• PDB: 7oba Cryo-EM structures of human RNA polymerase I.
• Chain: A
• Resolution: 3.1 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.6: DNA-directed RNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C64 C67 H77	C60 C63 H73
BS02	ZN	A	C107 C205	C103 C201

Gene Ontology

Molecular Function

• GO:0000287 magnesium ion binding
• GO:0001054 RNA polymerase I activity
• GO:0003677 DNA binding
• GO:0003682 chromatin binding
• GO:0003899 DNA-directed 5'-3' RNA polymerase activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016779 nucleotidyltransferase activity
• GO:0034062 5'-3' RNA polymerase activity
• GO:0046872 metal ion binding
• GO:0071667 DNA/RNA hybrid binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0006360 transcription by RNA polymerase I
• GO:1904750 negative regulation of protein localization to nucleolus

Cellular Component

• GO:0000428 DNA-directed RNA polymerase complex
• GO:0005634 nucleus
• GO:0005654 nucleoplasm
• GO:0005694 chromosome
• GO:0005730 nucleolus
• GO:0005736 RNA polymerase I complex

External links

• PDB: RCSB:7oba, PDBe:7oba, PDBj:7oba
• PDBsum: 7oba
• PubMed: 34887565
• UniProt: O95602|RPA1_HUMAN DNA-directed RNA polymerase I subunit RPA1 (Gene Name=POLR1A)