Structure of PDB 7nu8 Chain A

Receptor sequence

>7nu8A (length=870) Species: 485 (Neisseria gonorrhoeae)

MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYPSGKLHM
GHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAW
TYDNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVY
RKNGTVNWDPVDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAE
ELLNDLDKLEHWPEQVKTMQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAK
FLQVYTTRPDTLMGATYVAVAAEHPLATAAAADKPELQAFIAECKAGSVA
EADMATMEKKGVPTGRYVVNPLNGDKLEVWIANYVLWGYGDGAVMAVPAH
DERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYGDKENGVLVNSGDLD
GLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
KCGNVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRE
TDTMDTFIESSWYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAIL
HLLYARFFTKLMRDEGLVNVDEPFERLLTQGMVVCETYYRENDKGGKDWI
NPADVELTFDSAVLKADGLPVVISGTEKMSKSKNNGVDPQELINAYGADT
ARLFMMFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGGAVKAFA
GNQDGLSKELKDLRHKLHSTTAKVSDDYGRRQQFNTAIAAVMELLNQYDK
TDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPT
VDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFM
EGKPAKKIIVVPGRLVNIVV

3D structure

• PDB: 7nu8 Partitioning of the initial catalytic steps of leucyl-tRNA synthetase is driven by an active site peptide-plane flip.
• Chain: A
• Resolution: 2.11 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.4: leucine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	USB	A	F41 P42 Y43 G51 H52 N55 D80 G543 G544 E546 H547 H551 Q580 M582 V583 M635	F41 P42 Y43 G51 H52 N55 D80 G543 G544 E546 H547 H551 Q580 M582 V583 M629
BS02	ATP	A	Y255 T256 T257 L336 V344 R353 R428	Y255 T256 T257 L336 V344 R353 R428
BS03	ZN	A	C449 C452 C490 C493	C449 C452 C490 C493

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0002161 aminoacyl-tRNA editing activity
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004823 leucine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006429 leucyl-tRNA aminoacylation
• GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7nu8, PDBe:7nu8, PDBj:7nu8
• PDBsum: 7nu8
• PubMed: 36038645
• UniProt: B4RNT1|SYL_NEIG2 Leucine--tRNA ligase (Gene Name=leuS)