Structure of PDB 7nu0 Chain A

Receptor sequence

>7nu0A (length=865) Species: 485 (Neisseria gonorrhoeae)

MQEHYQPAAIEPAAQKKWDDARISNVSEDASKPKYYCLSMFPYPSGKLHM
GHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAW
TYDNIEYMKTQLKSLGFAVDWEREVATCKPEYYRWEQWLFTKLFEKGIVY
RKNGTVNWDPVDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAE
ELLNDLDKLEHWPEQVKTMQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAK
FLQVYTTRPDTLMGATYVAVAAEHPLATAAAADKPELQAFIAECKMEKKG
VPTGRYVVNPLNGDKLEVWIANYVLWGYGDGAVMAVPAHDERDFEFAAKY
NLPKKQVIAVGDNAFDANRWQEWYGDKENGVLVNSGDLDGLDFQTAFDAV
AAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCEKCGNVPVPADQ
LPVVLPENVVPDGMGSPLAKMPEFYETSCPCCGGAAKRETDTMDTFIESS
WYFFRYMSPKFSDGMVSAESAKYWGAVDQYIGGIEHAIAHLLYARFFTKL
MRDEGLVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDD
KGRPVSAVLKADGLPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFM
MFAAPPEQSLEWSDSGVEGAHRFLRRLWRTVYEYLKQGGAVKAFAGNQDG
LSKELKDLRHKLHSTTAKVSDDYGRRQQFNTAIAAVMELLNQYDKTDTGS
EQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAKLWEAGWPTVDEAA
LVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALANEGAVKFMEGKPA
KKIIVVPGRLVNIVV

3D structure

• PDB: 7nu0 Partitioning of the initial catalytic steps of leucyl-tRNA synthetase is driven by an active site peptide-plane flip.
• Chain: A
• Resolution: 1.89 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.4: leucine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ATP	A	P42 Y43 S45 H49 G51 H52 N55 Y56 R178 G544 E546 H547 Q580 M582 V583 K634 M635 K637 S638	P42 Y43 S45 H49 G51 H52 N55 Y56 R178 G533 E535 H536 Q569 M571 V572 K623 M624 K626 S627
BS02	DCL	A	F41 Y43 D80 S510 H547 H551	F41 Y43 D80 S499 H536 H540
BS03	ZN	A	C449 C452 C490 C493	C438 C441 C479 C482

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0002161 aminoacyl-tRNA editing activity
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004823 leucine-tRNA ligase activity
• GO:0005524 ATP binding

Biological Process

• GO:0006412 translation
• GO:0006418 tRNA aminoacylation for protein translation
• GO:0006429 leucyl-tRNA aminoacylation
• GO:0106074 aminoacyl-tRNA metabolism involved in translational fidelity

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7nu0, PDBe:7nu0, PDBj:7nu0
• PDBsum: 7nu0
• PubMed: 36038645
• UniProt: B4RNT1|SYL_NEIG2 Leucine--tRNA ligase (Gene Name=leuS)