Structure of PDB 7ntm Chain A

Receptor sequence

>7ntmA (length=347) Species: 559292 (Saccharomyces cerevisiae S288C)

SIPETQKGVIFYESHGKLEYKDIPVPKPKANELLINVKYSGVCHTDLHAW
HGDWPLPVKLPLVGGHEGAGVVVGMGENVKGWKIGDYAGIKWLNGSCMAC
EYCELGNESNCPHADLSGYTHDGSFQQYATADAVQAAHIPQGTDLAQVAP
ILCAGITVYKALKSANLMAGHWVAISGAAGGLGSLAVQYAKAMGYRVLGI
DGGEGKEELFRSIGGEVFIDFTKEKDIVGAVLKATDGGAHGVINVSVSEA
AIEASTRYVRANGTTVLVGMPAGAKCCSDVFNQVVKSISIVGSYVGNRAD
TREALDFFARGLVKSPIKVVGLSTLPEIYEKMEKGQIVGRYVVDTSK

3D structure

• PDB: 7ntm Cryo-EM structure of S.cerevisiae native alcohol dehydrogenase 1 (ADH1) in its tetrameric apo state
• Chain: A
• Resolution: 2.86 Å

Enzymatic activity

• Enzyme Commision number: 1.1.1.1: alcohol dehydrogenase.
  1.1.1.54: allyl-alcohol dehydrogenase.
  1.1.1.78: methylglyoxal reductase (NADH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	A	C43 H66	C43 H66
BS02	ZN	A	C97 C111	C97 C111

Gene Ontology

Molecular Function

• GO:0004022 alcohol dehydrogenase (NAD+) activity
• GO:0004552 octanol dehydrogenase (NAD+) activity
• GO:0005515 protein binding
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0019170 methylglyoxal reductase (NADH) activity
• GO:0042802 identical protein binding
• GO:0046872 metal ion binding
• GO:0047655 allyl-alcohol dehydrogenase activity
• GO:1904408 melatonin binding
• GO:1990362 butanol dehydrogenase (NAD+) activity

Biological Process

• GO:0000947 amino acid catabolic process to alcohol via Ehrlich pathway
• GO:0006116 NADH oxidation
• GO:0019655 glycolytic fermentation to ethanol

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:7ntm, PDBe:7ntm, PDBj:7ntm
• PDBsum: 7ntm
• UniProt: P00330|ADH1_YEAST Alcohol dehydrogenase 1 (Gene Name=ADH1)