Structure of PDB 7nsn Chain A

Receptor sequence

>7nsnA (length=1371) Species: 220687 (Neobacillus novalis)

SKTDFFSSFEKSDLQLTWTNTVETDANGKKMSSGIDGNVKRDLILGDITD
KVVQVTASANNPPNEIDSKLIDGDPTTKWLAFEPTANIVLKLAEPVAVVK
YALTSANDAKGRDPKNWTLYGSLDGTNWTAVDTREGEDFKDRFQRNMYDL
KNTTKYLYYKLDITKNAGDSITQLAEISLSDGIEVPAPPPGDMKSLIGKG
PTSSYTAKTNVGWTGLGALNYSGTHLSDGRAYSYNKLYDVDILVTPATEL
SYFIAPEFTDKNHNDYSSTYVSVDLAFSDGTYLHDLKAVDQYGVGLNPKD
QGDSKYLYVNQWNTIKSTIGSVAAGKTIKRILVAYDNPKGPGAFRGSIDD
IKIDGKPVQKAFGSPIDYVNILRGTQSNGSFSRGNNFPAVAIPHGFNFWT
PTTNAGSSWIYQYHESNSVNNLPQIQAFSVSHEPSPWMGDRQTFQVMPSA
STAATPNANRDSRALEFNHANEIAQPHYYSVKFENGIRTEMTPTDHAAMF
KFTFTGATSNLIFDNVNNNGGLTIDAKSGEITGYSDVKSGLSTGATRLFV
YAAFDKPVIKSGKLTGESRNNVTGYVRFDTSKDEDKVVTMKIATSLISVE
QAKKNLEQEIGLNDTFEGLKEKAKTEWNKKLGIIEVEGASEDQLVTLYSN
LYRLFLYPNSAFENVGTTTDPVYKYASPYSAATGQDTATTTGAKIVDGKT
YVNNGFWDTYRTAWPAYSLLTPTFAGELIDGFVQQYRDGGWIARWSSPGF
ANLMPGTSSDVAFADAYLKGVTNFDVQSFYQSAIRNAEAVSPNAGTGRKG
LTTSIFDGYTNTSTGEGLAWAMDGYINDFGIANLAKALKEKGDKSDPYYA
NYAADYQYFLNRAQNYVHMFNPSIEFFNGRTANGAWRSTPDNFNPAVWGS
DYTETNGWNMAFHVPQDGQGLANLYGGKEGLATKLDQFFSTSETGLFPGS
YGGTIHEMREARDVRMGMYGHSNQPSHHIAYMYDYAGQPWKTQEKVREAL
NRLYIGSAIGQGYSGDEDNGEMSAWYILSAMGFYPLKMGTPEYAIGAPLF
KKATIHLENGKSIVINAPNNSKENKYVQSMKVNGKAYAKTSILHADIANG
AVIDFEMGSKPSKWGSGDQDILQSITPGSTDGTSLSPLPLRDVTDRLIAA
EKGAVTVSDEGNGQLLFDNTSNTQLSMKSKTPSIVYQFKEGKQNVKMYTL
TSSKASQNEDPKSWVLKGSNDGKSWSVLDQRKNETFQWRQYTRAFTIQHP
GKYSQYKLEITENAGAEVTTLAELELLGYDDVTNSYQAVYELMEQFKQSK
DLTGPMAVQLNNSLTTSLDHFKKDHKDQAIKHLEDFLKHLNNKGLQDRIS
SKAKGVLSADANQLIVLLARD

3D structure

• PDB: 7nsn Structural and functional characterization of multi-domain GH92 alpha-1,2-mannosidase from Neobacillus novalis
• Chain: A
• Resolution: 2.29 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	A	N1013 Q1014 D1058	N973 Q974 D1018
BS02	CA	A	S48 E50 T254 G257 D389	S8 E10 T214 G217 D349
BS03	CA	A	D1182 D1185 N1209	D1142 D1145 N1169
BS04	CA	A	L1205 D1208 T1210 T1213 A1312 E1313	L1165 D1168 T1170 T1173 A1272 E1273
BS05	MVL	A	S475 W477 W747 D748 W785 M794 E944 D1058	S435 W437 W707 D708 W745 M754 E904 D1018
BS06	CA	A	K109 D112 D114 T117 A215 E216	K69 D72 D74 T77 A175 E176
BS07	MVL	A	E105 K118 D148 R152	E65 K78 D108 R112

Gene Ontology

Molecular Function

• GO:0030246 carbohydrate binding

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:7nsn, PDBe:7nsn, PDBj:7nsn
• PDBsum: 7nsn
• PubMed: 37071393
• UniProt: A0A7R9KAR8