Structure of PDB 7niw Chain A

Receptor sequence

>7niwA (length=1140) Species: 9606 (Homo sapiens)

TLFRYSDWQDKLFMSLGTIMAIAHGSGLPLMMIVFGEMTDKFVDTAGNFS
FPVPGKILEEEMTRYAYYYSGLGAGVLVAAYIQVSFWTLAAGRQIRKIRQ
KFFHAILRQEIGWFDINDTTELNTRLTDDISKISEGIGDKVGMFFQAVAT
FFAGFIVGFIRGWKLTLVIMAISPILGLSAAVWAKILSAFSDKELAAYAK
AGAVAEEALGAIRTVIAFGGQNKELERYQKHLENAKEIGIKKAISANISM
GIAFLLIYASYALAFWYGSTLVISKEYTIGNAMTVFFSILIGAFSVGQAA
PCIDAFANARGAAYVIFDIIDNNPKIDSFSERGHKPDSIKGNLEFNDVHF
SYPSRANVKILKGLNLKVQSGQTVALVGSSGCGKSTTVQLIQRLYDPDEG
TINIDGQDIRNFNVNYLREIIGVVSQEPVLFSTTIAENICYGRGNVTMDE
IKKAVKEANAYEFIMKLPQKFDTLVGERGAQLSGGQKQRIAIARALVRNP
KILLLDQATSALDTESEAEVQAALDKAREGRTTIVIAHRLSTVRNADVIA
GFEDGVIVEQGSHSELMKKEGVYFKLVNANVPPVSFLKVLKLNKTEWPYF
VVGTVCAIANGGLQPAFSVIFSEIIAIFGPGDDAVKQQKCNIFSLIFLFL
GIISFFTFFLQGFTFGKAGEILTRRLRSMAFKAMLRQDMSWFDDHKNSTG
ALSTRLATDAAQVQGATGTRLALIAQNIANLGTGIIISFIYGWQLTLLLL
AVVPIIAVSGIVEMKLLAGNAKRDKKELEAAGKIATEAIENIRTVVSLTQ
ERKFESMYVEKLYGPYRNSVQKAHIYGITFSISQAFMYFSYAGCFRFGAY
LIVNGHMRFRDVILVFSAIVFGAVALGHASSFAPDYAKAKLSAAHLFMLF
ERQPLIDSYSEEGLKPDKFEGNITFNEVVFNYPTRANVPVLQGLSLEVKK
GQTLALVGSSGCGKSTVVQLLERFYDPLAGTVLLDGQEAKKLNVQWLRAQ
LGIVSQEPILFDCSIAENIAYGDNSRVVSQDEIVSAAKAANIHPFIETLP
HKYETRVGDKGTQLSGGQKQRIAIARALIRQPQILLLDQATSALDTESEK
VVQEALDKAREGRTCIVIAHRLSTIQNADLIVVFQNGRVK

3D structure

• PDB: 7niw Structures of ABCB4 provide insight into phosphatidylcholine translocation.
• Chain: A
• Resolution: 3.8 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.1: P-type phospholipid transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CLR	A	S121 F950	S70 F839
BS02	CLR	A	W317 N752 L756 L759	W266 N641 L645 L648
BS03	CLR	A	F203 I211	F152 I160
BS04	X2N	A	M301 I342 F345 F769 Q772 Q837 Y952 F982 V985 H989 F993	M250 I291 F294 F658 Q661 Q726 Y841 F871 V874 H878 F882
BS05	DLP	A	S224 W234 M301 F305 I308 Y312 F337 F345 Q349 F728 F993	S173 W183 M250 F254 I257 Y261 F286 F294 Q298 F617 F882

Gene Ontology

Molecular Function

• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0005548 phospholipid transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0090554 phosphatidylcholine floppase activity
• GO:0099038 ceramide floppase activity
• GO:0140326 ATPase-coupled intramembrane lipid transporter activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0006629 lipid metabolic process
• GO:0006869 lipid transport
• GO:0032376 positive regulation of cholesterol transport
• GO:0032782 bile acid secretion
• GO:0045332 phospholipid translocation
• GO:0055085 transmembrane transport
• GO:0055088 lipid homeostasis
• GO:0061092 positive regulation of phospholipid translocation
• GO:0099040 ceramide translocation
• GO:1901557 response to fenofibrate
• GO:1903413 cellular response to bile acid
• GO:2001140 positive regulation of phospholipid transport

Cellular Component

• GO:0005654 nucleoplasm
• GO:0005737 cytoplasm
• GO:0005829 cytosol
• GO:0005886 plasma membrane
• GO:0005925 focal adhesion
• GO:0016020 membrane
• GO:0016324 apical plasma membrane
• GO:0030136 clathrin-coated vesicle
• GO:0031410 cytoplasmic vesicle
• GO:0045121 membrane raft
• GO:0046581 intercellular canaliculus
• GO:0070062 extracellular exosome

External links

• PDB: RCSB:7niw, PDBe:7niw, PDBj:7niw
• PDBsum: 7niw
• PubMed: 34385322
• UniProt: P21439|MDR3_HUMAN Phosphatidylcholine translocator ABCB4 (Gene Name=ABCB4)