Structure of PDB 7nis Chain A

Receptor sequence

>7nisA (length=513) Species: 88776 (Influenza A virus (A/Brevig Mission/1/1918(H1N1)))

RFEITGTMRRLADQSLPPNFSSLENFRAYVDGFEPNGYIEGKLSQMSKEV
NARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIP
LYDAIKCMRTFFGWKEPNVVKPHEKGINPNYLLAWKQVLAELQDIENEEK
IPKTKNMKKTSQLKWALGENMAPEKVDFDDCKDVSDLKQYDSDEPELRSL
ASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHC
RATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGF
IIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHKWEKYCVLEIGDMLLRSA
IGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCLLQSLQQIESMIEAESSVK
EKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASP
QLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLN
ASWFNSFLTHALR

3D structure

• PDB: 7nis Mapping inhibitory sites on the RNA polymerase of the 1918 pandemic influenza virus using nanobodies.
• Chain: A
• Resolution: 5.96 Å

Enzymatic activity

• Enzyme Commision number: 3.1.-.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	R279 H326 K328 G371 M374 A375 P376 Y393 D394 E397 H510 D516 V517 N519 K539 R566 T567 N568 K574 P653	R76 H123 K125 G168 M171 A172 P173 Y190 D191 E194 H307 D313 V314 N316 K336 R363 T364 N365 K371 P450

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0004519 endonuclease activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006351 DNA-templated transcription
• GO:0039523 symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity
• GO:0039694 viral RNA genome replication
• GO:0075523 viral translational frameshifting
• GO:0075526 cap snatching

Cellular Component

• GO:0030430 host cell cytoplasm
• GO:0042025 host cell nucleus

External links

• PDB: RCSB:7nis, PDBe:7nis, PDBj:7nis
• PDBsum: 7nis
• PubMed: 35017564
• UniProt: Q3HM39|PA_I18A0 Polymerase acidic protein (Gene Name=PA)