Structure of PDB 7nac Chain A

Receptor sequence

>7nacA (length=266) Species: 1247190 (Saccharomyces cerevisiae BY4741)

KQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSRKEPKLDTKKDLQQL
NEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTMDELN
FTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFID
HVMSFSIVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVI
LILEGSFGGPKIYENKQYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKR
RENVLAADPLSNDALF

3D structure

• PDB: 7nac Sequence-specific remodeling of a topologically complex RNP substrate by Spb4.
• Chain: A
• Resolution: 3.04 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	N42 R46 K68 R92 K93 H94 N246 V248 Q251 I252 Q255 E259 R263 R270	N18 R22 K44 R68 K69 H70 N222 V224 Q227 I228 Q231 E235 R239 R246

Gene Ontology

Molecular Function

• GO:0003723 RNA binding
• GO:0005515 protein binding
• GO:0008097 5S rRNA binding
• GO:0019843 rRNA binding
• GO:0042134 rRNA primary transcript binding

Biological Process

• GO:0000027 ribosomal large subunit assembly
• GO:0000464 endonucleolytic cleavage in ITS1 upstream of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0000465 exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
• GO:0006364 rRNA processing
• GO:0042254 ribosome biogenesis

Cellular Component

• GO:0005634 nucleus
• GO:0005730 nucleolus
• GO:0030687 preribosome, large subunit precursor

External links

• PDB: RCSB:7nac, PDBe:7nac, PDBj:7nac
• PDBsum: 7nac
• PubMed: 36482249
• UniProt: Q08235|BRX1_YEAST Ribosome biogenesis protein BRX1 (Gene Name=BRX1)