Structure of PDB 7n2m Chain A

Receptor sequence

>7n2mA (length=865) Species: 9606 (Homo sapiens)

EQVFHFYWLDAYEDQYNQPGVVFLFGKVWIESAETHVSCCVMVKNIERTL
YFLPREMKIDLNTGKETGTPISMKDVYEEFDEKIATKYKIMKFKSKPVEK
NYAFEIPDVPEKSEYLEVKYSAEMPQLPQDLKGETFSHVFGTNTSSLELF
LMNRKIKGPCWLEVKSPQLLNQPVSWCKAEAMALKPDLVNVIKDVSPPPL
VVMAFSMKTMQNAKNHQNEIIAMAALVHHSFALDKAAPKPPFQSHFCVVS
KPKDCIFPYAFKEVIEKKNVKVEVAATERTLLGFFLAKVHKIDPDIIVGH
NIYGFELEVLLQRINVCKAPHWSKIGRLKRSNMPKLGFGERNATCGRMIC
DVEISAKELIRCKSYHLSELVQQILKTERVVIPMENIQNMYSESSQLLYL
LEHTWKDAKFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFL
LLHAFYENNYIVPDKQIRKKAAYAGGLVLDPKVGFYDKFILLLDFNSLYP
SIIQEFNICFTTVQRVEQIPELPDPSLEMGILPREIRKLVERRKQVKQLM
KQQDLNPDLILQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYK
GREILMHTKEMVQKMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEV
NKLYKLLEIDIDGVFKSLLLLKKKKYAALVVEPTSDGNYVTKQELKGLDI
VRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEIGENVLNGSVP
VSQFEINKALTKDPQDYPDKKSLPHVHVALWINSQGGRKVKAGDTVSYVI
CQDGSNLTASQRAYAPEQLQKQDNLTIDTQYYLAQQIHPVVARICEPIDG
IDAVLIATWLGLDPT

3D structure

• PDB: 7n2m Structural and functional insight into mismatch extension by human DNA polymerase alpha.
• Chain: A
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	D1002 K1053 K1075 G1076 R1081 R1082 D1083 A1138 T1140 Y1146 H1154	D623 K674 K696 G697 R702 R703 D704 A759 T761 Y767 H775
BS02	dna	A	G783 R784 S785 R834 A837 Y839 G841 G842 V844 S955 Y957 G958 G961 F962 Y964 K1051 K1052 K1053 T1187 S1189 R1222	G442 R443 S444 R468 A471 Y473 G475 G476 V478 S576 Y578 G579 G582 F583 Y585 K672 K673 K674 T808 S810 R843
BS03	DCP	A	D860 F861 N862 S863 L864 Y865 R922 K950 N954 Y957 D1004	D494 F495 N496 S497 L498 Y499 R543 K571 N575 Y578 D625
BS04	MG	A	D860 F861 D1004	D494 F495 D625
BS05	ZN	A	D860 D1004	D494 D625
BS06	ZN	A	H342 E500	H5 E163

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:1902975 mitotic DNA replication initiation

External links

• PDB: RCSB:7n2m, PDBe:7n2m, PDBj:7n2m
• PDBsum: 7n2m
• PubMed: 35467978
• UniProt: P09884|DPOLA_HUMAN DNA polymerase alpha catalytic subunit (Gene Name=POLA1)