Structure of PDB 7mym Chain A

Receptor sequence

>7mymA (length=158) Species: 562 (Escherichia coli)

MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTWESI
GRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVY
EQFLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHS
YCFEILER

3D structure

• PDB: 7mym Structure-guided functional studies of plasmid-encoded dihydrofolate reductases reveal a common mechanism of trimethoprim resistance in Gram-negative pathogens.
• Chain: A
• Resolution: 3.04 Å

Enzymatic activity

• Enzyme Commision number: 1.5.1.3: dihydrofolate reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ARG	A	D131 D132 W133 R158	D131 D132 W133 R158
BS02	NAP	A	A7 I14 G15 N18 A19 M20 G43 R44 H45 T46 L62 S63 S64 K76 I94 G96 G97 R98 V99 Q102 T123	A7 I14 G15 N18 A19 M20 G43 R44 H45 T46 L62 S63 S64 K76 I94 G96 G97 R98 V99 Q102 T123
BS03	TOP	A	I5 A6 M20 D27 L28 F31 I50 I94	I5 A6 M20 D27 L28 F31 I50 I94

Gene Ontology

Molecular Function

• GO:0004146 dihydrofolate reductase activity
• GO:0005515 protein binding
• GO:0005542 folic acid binding
• GO:0016491 oxidoreductase activity
• GO:0050661 NADP binding
• GO:0051870 methotrexate binding
• GO:0051871 dihydrofolic acid binding
• GO:0070401 NADP+ binding
• GO:0070402 NADPH binding

Biological Process

• GO:0006730 one-carbon metabolic process
• GO:0009257 10-formyltetrahydrofolate biosynthetic process
• GO:0009410 response to xenobiotic stimulus
• GO:0031427 response to methotrexate
• GO:0046452 dihydrofolate metabolic process
• GO:0046654 tetrahydrofolate biosynthetic process
• GO:0046655 folic acid metabolic process
• GO:0046656 folic acid biosynthetic process
• GO:0046677 response to antibiotic

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:7mym, PDBe:7mym, PDBj:7mym
• PDBsum: 7mym
• PubMed: 35562546
• UniProt: P0ABQ4|DYR_ECOLI Dihydrofolate reductase (Gene Name=folA)