Structure of PDB 7mwu Chain A

Receptor sequence

>7mwuA (length=499) Species: 562 (Escherichia coli)

QSVSRAAITAAYRRPETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRN
QKNASGRAGMVQGLLQEFSLSSQEGVALMCLAEALLRIPDKATRDALIRD
KILFVNAATWGLLFASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVT
GETIAEALANARKLEEKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAI
GKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEELYPRLKSLTLLAR
QYDIGINIDAEESDRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCP
LVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVY
TDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQYEFQ
CLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENG
ANTSFVNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRD

3D structure

• PDB: 7mwu Structure-affinity relationships of reversible proline analog inhibitors targeting proline dehydrogenase.
• Chain: A
• Resolution: 1.69 Å

Enzymatic activity

• Enzyme Commision number: 1.2.1.88: L-glutamate gamma-semialdehyde dehydrogenase.
  1.5.5.2: proline dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	A	D370 A371 V402 Q404 R431 V433 K434 G435 A436 Y437 W438 Y456 T457 R458 K459 T462 A485 T486 H487 N488 C512 L513 E559 S565	D259 A260 V291 Q293 R320 V322 K323 G324 A325 Y326 W327 Y345 T346 R347 K348 T351 A374 T375 H376 N377 C401 L402 E448 S454
BS02	ZPM	A	K329 L513 Y540 Y552 R555 R556	K218 L402 Y429 Y441 R444 R445

Gene Ontology

Molecular Function

• GO:0003842 1-pyrroline-5-carboxylate dehydrogenase activity
• GO:0004657 proline dehydrogenase activity

Biological Process

• GO:0006562 proline catabolic process

External links

• PDB: RCSB:7mwu, PDBe:7mwu, PDBj:7mwu
• PDBsum: 7mwu
• PubMed: 35018940
• UniProt: P09546|PUTA_ECOLI Bifunctional protein PutA (Gene Name=putA)