Structure of PDB 7mix Chain A

Receptor sequence

>7mixA (length=1326) Species: 9606 (Homo sapiens)

KYAKRITEWPPFEYMILATIIANCIVLALEQHLPDGDKTPMSERLDDTEP
YFIGIFCFEAGIKIIALGFVSYLRNGWNVMDFVVVLTGILATAGTDFDLR
TLRAVRVLRPLKLVSGIPSLQVVLKSIMKAMVPLLQIGLLLFFAILMFAI
IGLEFYMGKFHKACFPNSTDAEPVGDFPCGKEAPARLCEGDTECREYWPG
PNFGITNFDNILFAILTVFQCITMEGWTDILYNTNDAAGNTWNWLYFIPL
IIIGSFFMLNLVLGVLSGEFAKERERVENRRAFLKLRRQQQIERELNGYL
EWIFKAEEVMLAEEDRNFRRKEKMFRFFIRRMVKAQSFYWVVLCVVALNT
LCVAMVHYNQPRRLTTTLYFAEFVFLGLFLTEMSLKMYGLGPRSYFRSSF
NCFDFGVIVGSVFEVVWAAIKPGSSFGISVLRALRLLRIFKVTKYWSSLR
NLVVSLLNSMKSIISLLFLLFLFIVVFALLGMQLFGGQFNFQDETPTTNF
DTFPAAILTVFQILTGEDWNAVMYHGIESQGGVSKGMFSSFYFIVLTLFG
NYTLLNVFLAIAVDNLANAQELTKDEEEMEEAANQKLALQKAKEVAEVSP
MSAANISIAARQQNSAKARSVWEQRASQLRLQNLRASCEALRRFCHYIVT
MRYFEVVILVVIALSSIALAAEDPVRTDSPRNNALKYLDYIFTGVFTFEM
VIKMIDLWNILDFIVVSGALVAFAFSGSKGKDINTIKSLRVLRVLRPLKT
IKRLPKLKAVFDCVVNSLKNVLNILIVYMLFMFIFAVIAVQLFKGKFFYC
TDESKELERDCRGQYLDYEKEEVEAQPRQWKKYDFHYDNVLWALLTLFTV
STGEGWPMVLKHSVDATYEEQGPSPGYRMELSIFYVVYFVVFPFFFVNIF
VALIIITFQEQGDKVMSECSLEKNERACIDFAISAKPLTRYMPQNRQSFQ
YKTWTFVVSPPFEYFIMAMIALNTVVLMMKFYDAPYEYELMLKCLNIVFT
SMFSMECVLKIIAFGVLNYFRDAWNVFDFVTVLGSITDILVTEIAETNNF
INLSFLRLFRAARLIKLLRQGYTIRILLWTFVQSFKALPYVCLLIAMLFF
IYAIIGMQVFGNIALDDDTSINRHNNFRTFLQALMLLFRSATGEAWHEIM
LSCLSNQACDEQANATECGSDFAYFYFVSFIFLCSFLMLNLFVAVIMDNF
EYLTRDSSILGPHHLDEFIRVWAEYDPAACGRISYNDMFEMLKHMSPPLG
LGKKCPARVAYKRLVRMNMPISNEDMTVHFTSTLMALIRTALEIKLAPAG
TKQHQCDAELRKEISVVWANLPQKTL

3D structure

• PDB: 7mix Structure of human Ca v 2.2 channel blocked by the painkiller ziconotide.
• Chain: A
• Resolution: 3.0 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	peptide	A	T641 P642 T643 T644 D664 Y1310 D1345 P1368 M1369 K1372 D1628 D1629 E1659	T495 P496 T497 T498 D518 Y799 D834 P857 M858 K861 D1117 D1118 E1148
BS02	CA	A	E314 E663 E1365	E225 E517 E854
BS03	Y01	A	F617 L654 M1390 S1393 I1394 V1397	F471 L508 M879 S882 I883 V886
BS04	CLR	A	F1292 F1296 E1391 I1394 V1398	F781 F785 E880 I883 V887
BS05	CLR	A	M1489 F1492	M978 F981
BS06	Y01	A	A500 H503	A354 H357
BS07	CLR	A	P1178 R1179 L1186	P680 R681 L688
BS08	CLR	A	S1666 S1681 D1682	S1155 S1170 D1171
BS09	PT5	A	S545 F546 L583 R584 K587 W592 R596 V599 F1406	S399 F400 L437 R438 K441 W446 R450 V453 F895

Gene Ontology

Molecular Function

• GO:0001540 amyloid-beta binding
• GO:0005216 monoatomic ion channel activity
• GO:0005245 voltage-gated calcium channel activity
• GO:0005262 calcium channel activity
• GO:0005509 calcium ion binding
• GO:0005515 protein binding
• GO:0005524 ATP binding
• GO:0008331 high voltage-gated calcium channel activity
• GO:0046872 metal ion binding

Biological Process

• GO:0006811 monoatomic ion transport
• GO:0006816 calcium ion transport
• GO:0007268 chemical synaptic transmission
• GO:0050804 modulation of chemical synaptic transmission
• GO:0055085 transmembrane transport
• GO:0070588 calcium ion transmembrane transport
• GO:0098703 calcium ion import across plasma membrane
• GO:1904645 response to amyloid-beta

Cellular Component

• GO:0005886 plasma membrane
• GO:0005891 voltage-gated calcium channel complex
• GO:0016020 membrane
• GO:0034702 monoatomic ion channel complex
• GO:0043025 neuronal cell body
• GO:0045202 synapse

External links

• PDB: RCSB:7mix, PDBe:7mix, PDBj:7mix
• PDBsum: 7mix
• PubMed: 34234349
• UniProt: Q00975|CAC1B_HUMAN Voltage-dependent N-type calcium channel subunit alpha-1B (Gene Name=CACNA1B)