Structure of PDB 7m5d Chain A

Receptor sequence

>7m5dA (length=360) Species: 562 (Escherichia coli)

MKSSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQLSDVSR
CFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLL
LPKDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMS
ASEGEHGGYKEIIAKICGDGVYGRLKFESGGHRVQRVPATESQGRIHTSA
CTVAVMPELPDAELPDINPADLRIDTFRSSGAGGQHVNTTDSAIRITHLP
TGIVVECQDERSQHKNKAKALSVLGARIHAAEMAKRQQAEASTRRNLLGS
GDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVMEGKLDMLIEPIIQEHQA
DQLAALSEQE

3D structure

• PDB: 7m5d Cryo-EM structure of a non-rotated E.coli 70S ribosome in complex with RF3-GTP, RF1 and P-tRNA (state I)
• Chain: A
• Resolution: 2.8 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	I29 Q32 R36 S39 R40 A43 R116 F227 R228 S230 G231 A232 G233 G234 Q235 H236 V237 N238 T239 T240 D241 R245 E256 D259 R261 Q263 H264 R294 R295 L298	I29 Q32 R36 S39 R40 A43 R116 F227 R228 S230 G231 A232 G233 G234 Q235 H236 V237 N238 T239 T240 D241 R245 E256 D259 R261 Q263 H264 R294 R295 L298
BS02	rna	A	G118 T119 R133 K160 R183 Q185 Q193 G301 R303 R306 Q314 R316	G118 T119 R133 K160 R183 Q185 Q193 G301 R303 R306 Q314 R316
BS03	rna	A	G120 P188 T190 R195 I196 H197 T198	G120 P188 T190 R195 I196 H197 T198
BS04	rna	A	E155 H156 G233 G234 E260 R261	E155 H156 G233 G234 E260 R261

Gene Ontology

Molecular Function

• GO:0003747 translation release factor activity
• GO:0005515 protein binding
• GO:0016149 translation release factor activity, codon specific
• GO:0043022 ribosome binding

Biological Process

• GO:0006412 translation
• GO:0006415 translational termination

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:7m5d, PDBe:7m5d, PDBj:7m5d
• PDBsum: 7m5d
• UniProt: P0A7I0|RF1_ECOLI Peptide chain release factor RF1 (Gene Name=prfA)