Structure of PDB 7m4l Chain A

Receptor sequence

>7m4lA (length=320) Species: 9606 (Homo sapiens)

NHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEAC
SIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQ
MWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQ
KAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDS
LRQEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLY
FTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPGRVLPTPT
EKDVFRLLGLPYREPAERDW

3D structure

• PDB: 7m4l Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.
• Chain: A
• Resolution: 1.701 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 G464 T471 K472 R514 R517 K521 L527 S528 E529 H530 H541	W24 V212 G214 T216 K217 R259 R262 K266 L272 S273 E274 H275 H286
BS02	dna	A	W342 G343 A344 G345 K347 T348 D429 D490 Y505 T507 G508 A510 N513	W92 G93 A94 G95 K97 T98 D179 D235 Y250 T252 G253 A255 N258
BS03	dna	A	Y267 W274 R275 Y279 P303 G304 G306 M309 K312	Y17 W24 R25 Y29 P53 G54 G56 M59 K62
BS04	MN	A	D427 D429	D177 D179
BS05	MN	A	D427 D429 D490	D177 D179 D235
BS06	MN	A	D382 H486	D132 H231
BS07	PPV	A	R386 G416 S417 R420 G426	R136 G166 S167 R170 G176

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7m4l, PDBe:7m4l, PDBj:7m4l
• PDBsum: 7m4l
• PubMed: 35680862
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)