Structure of PDB 7m4i Chain A

Receptor sequence

>7m4iA (length=318) Species: 9606 (Homo sapiens) [Search protein sequence]

HNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEACS
IPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQM
WYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQK
AAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSL
RQEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYF
TGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNHGAKVGPGRVLPTPTEK
DVFRLLGLPYREPAERDW

3D structure

PDB

7m4i Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.

Chain

Resolution

2.005 Å

3D
structure

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Enzymatic activity

Enzyme Commision number

2.7.7.7: DNA-directed DNA polymerase.
4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	dna	A	W274 V462 G464 E470 Y505 R517 K521 H530	W23 V211 G213 E214 Y249 R261 K265 H274
BS02	dna	A	W342 G343 G345 K347 T348 D427 D429 Y505 F506 T507 G508 A510 N513	W91 G92 G94 K96 T97 D176 D178 Y249 F250 T251 G252 A254 N257
BS03	dna	A	W274 R275 Y279 P303 G304 G306 R308 M309 K312	W23 R24 Y28 P52 G53 G55 R57 M58 K61
BS04	MN	A	D427 D429	D176 D178
BS05	MN	A	D427 D429 D490	D176 D178 D234
BS06	MN	A	D382 H486	D131 H230
BS07	PPV	A	S417 R420 G426 D427 D429	S166 R169 G175 D176 D178

Gene Ontology

	Molecular Function
GO:0003677	DNA binding
GO:0003887	DNA-directed DNA polymerase activity
GO:0016779	nucleotidyltransferase activity
GO:0034061	DNA polymerase activity

	Biological Process
GO:0006281	DNA repair

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Molecular Function

View graph for
Biological Process

External links

PDB	RCSB:7m4i, PDBe:7m4i, PDBj:7m4i
PDBsum	7m4i
PubMed	35680862
UniProt	Q9UGP5\|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)

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