Structure of PDB 7m4b Chain A

Receptor sequence

>7m4bA (length=320) Species: 9606 (Homo sapiens)

NHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKSFHKPVTSYQEAC
SIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELFSNIWGAGTKTAQ
MWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMPREEATEIEQTVQ
KAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDS
LRQEGFLTDDLVKGETKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLY
FTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGAKVGPGRVLPTPT
EKDVFRLLGLPYREPAERDW

3D structure

• PDB: 7m4b Watching right and wrong nucleotide insertion captures hidden polymerase fidelity checkpoints.
• Chain: A
• Resolution: 1.66 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 G464 T471 K472 R514 R517 K521 L527 S528 E529 H530 H541	W24 V212 G214 T216 K217 R259 R262 K266 L272 S273 E274 H275 H286
BS02	dna	A	W342 G343 A344 G345 K347 T348 D427 D429 R488 D490 Y505 T507 G508 A510 N513	W92 G93 A94 G95 K97 T98 D177 D179 R233 D235 Y250 T252 G253 A255 N258
BS03	dna	A	Y267 W274 R275 Y279 P303 G304 G306 R308 M309 K312	Y17 W24 R25 Y29 P53 G54 G56 R58 M59 K62
BS04	MN	A	D427 D429	D177 D179
BS05	MN	A	D427 D429 D490	D177 D179 D235
BS06	MN	A	D382 R485 H486	D132 R230 H231
BS07	PPV	A	R386 G416 S417 R420 G426	R136 G166 S167 R170 G176

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7m4b, PDBe:7m4b, PDBj:7m4b
• PDBsum: 7m4b
• PubMed: 35680862
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)