Structure of PDB 7m07 Chain A

Receptor sequence

>7m07A (length=327) Species: 9606 (Homo sapiens)

WVCAQPSSQKATNHNLHITEKLEVLAKAYSVQGDKWRALGYAKAINALKS
FHKPVTSYQEACSIPGIGKRMAEKIIEILESGHLRKLDHISESVPVLELF
SNIWGAGTKTAQMWYQQGFRSLEDIRSQASLTTQQAIGLKHYSDFLERMP
REEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGR
SHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRR
LDIIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVPG
RVLPTPTEKDVFRLLGLPYREPAERDW

3D structure

• PDB: 7m07 Analysis of diverse double-strand break synapsis with Pol lambda reveals basis for unique substrate specificity in nonhomologous end-joining.
• Chain: A
• Resolution: 1.57 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.
  4.2.99.-

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W274 V462 S463 Q464 Q471 K472 R514 R517 K521 L527 S528 E529 H530	W36 V224 S225 Q226 Q233 K234 R276 R279 K283 L289 S290 E291 H292
BS02	dna	A	W342 G343 A344 G345 K347 T348 D490 Y505	W104 G105 A106 G107 K109 T110 D252 Y267
BS03	dna	A	Y267 W274 R275 Y279 P303 G304 G306 M309 K312	Y29 W36 R37 Y41 P65 G66 G68 M71 K74
BS04	DUP	A	R386 G416 S417 R420 G426 D427 D429 Y505 T507 G508 A510 N513	R148 G178 S179 R182 G188 D189 D191 Y267 T269 G270 A272 N275
BS05	MG	A	D427 D429 D490	D189 D191 D252
BS06	MG	A	D427 D429	D189 D191

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:7m07, PDBe:7m07, PDBj:7m07
• PDBsum: 7m07
• PubMed: 35778389
• UniProt: Q9UGP5|DPOLL_HUMAN DNA polymerase lambda (Gene Name=POLL)