Structure of PDB 7lvv Chain A

Receptor sequence

>7lvvA (length=1011) Species: 980427 (Deinococcus wulumuqiensis)

SLQLVKKFQKRLEDIVAYGGTRNESSVRAAFQQLLSDWAEGSGLRLITEV
TQKAVAGNNVRPDGTLKDSLQQSRGYWESKDEADTLDDEIQKKLAKGYPR
DNIIFEDSRLAVLMQNGEEVQRVDMGDAGALAGLLKLFFEFEPPQVLEFR
KAVDHFKDEMPHLLKILREAADAAEQKADYRGERDHFVEIAKEAINPDFS
PRDAREMLIQHILTGDLFTSVFDNAQYHEDNNIAQQLQQLAATFYKGPVK
RDIAERTKRYYGAIQAAAAQIADHHEKQRFLKALYENFYRAYNPAGAERL
GIFYTPGEIVRFMIEATDTLLEKHFQKELADKGVEILDPATGTGTFITEL
IDFLPKAKLEQKYREELHCNELALLPYYIANLNIEATYAQKMGRYEEFRN
IVLVDTLDNTLFGSVTAENLERAKRQNARPVRVIIGNPPYRANQANENDN
NKNREYKEIDRRIKATYVAASTAQKTKLYDMYSRFLRWATDRLKEDGIVA
FVSNSSFIDSRTFDGFRKEVVKDFDHIYILDMKGNANTSGERRKREGGNV
FNDQIKVGVAVYFLVRSADTKIWYHAVPDFWRAREKLEWLKTTKFEDIEF
DHIRPDAKHNWLGQVDEENDWNEFLPVADKDTKQAKGLGQERAIFKLYSL
GVVTNRDEWVYSRAEDELADKVRYFIGRYNEIIKLPLGDLMSRNWEGDIK
MTRATIADAQSRKSYSLEKNSIVPSLYRPFDVLKMYFSKNLNEMQYQMPS
IFPKGVGENVVIALSGSPAAKPFQVLATDILPSLDLLEKTQCLPFYRYTM
NGERLNNITDYALKAFQTHYADTSISREDIFHYVYAVLHHPAYREKYALN
LRQEFPRIPFYPEFGRWAAWGRELMALHIGFESVAPYPLKRTDEPPKNDT
PEALALAKKARLKVQRDAAKQPTGAVELDGLTTLAGIPAAAWAYKLGNRS
ALEWVLERHKETTPKDATIREKFNTYRFADHKERVIDLLARVTTVSVETV
RIVGEMPAETM

3D structure

• PDB: 7lvv Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM.
• Chain: A
• Resolution: 3.25 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	G302 F304 P449 Y451 A453 A484 Q485 K486 K488 N515 S521 R522 D564 Q565 I566 K567 V568 L668 M762 Y764 Q765	G301 F303 P438 Y440 A442 A473 Q474 K475 K477 N504 S510 R511 D553 Q554 I555 K556 V557 L650 M744 Y746 Q747
BS02	dna	A	N454 Q455 E458 K463 Q485 T487 Y490 N548 S550 R554 K567 K651 N673 D675 R721 R746 D803	N443 Q444 E447 K452 Q474 T476 Y479 N537 S539 R543 K556 K633 N655 D657 R703 R728 D785
BS03	dna	A	N24 E25 S26 R62	N23 E24 S25 R61
BS04	SAM	A	Y286 L301 F304 A341 G343 T344 F347 E372 L373 D406 T407 P450 F496	Y285 L300 F303 A340 G342 T343 F346 E371 L372 D405 T406 P439 F485
BS05	CA	A	E25 D64 E79 S80	E24 D63 E78 S79

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0008168 methyltransferase activity
• GO:0008170 N-methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:7lvv, PDBe:7lvv, PDBj:7lvv
• PDBsum: 7lvv
• PubMed: 33826880
• UniProt: A0A345IJ72