Structure of PDB 7lsk Chain A

Receptor sequence

>7lskA (length=554) Species: 11676 (Human immunodeficiency virus 1)

PISPIETVPVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKI
GPENPYNTPVFAIKKKDSTKWRKLVDFRELNKRTQDFWEVQLGIPHPAGL
KKKKSVTVLDVGDAYFSVPLDEDFRKYTAFTIPSINNETPGIRYQYNVLP
QGWKGSPAIFQSSMTKILEPFKKQNPDIVIYQYMDDLYVGSDLEIGQHRT
KIEELRQHLLRWGLTTPDKKHQKEPPFLWMGYELHPDKWTVQPIVLPEKD
SWTVNDIQKLVGKLNWASQIYPGIKVRQLSKLLRGTKALTEVIPLTEEAE
LELAENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLK
TGKYARMRGAHTNDVKQLTEAVQKITTESIVIWGKTPKFKLPIQKETWET
WWTEYWQATWIPEWEFVNTPPLVKLWYQLEKEPIVGAETFYVDGAANRET
KLGKAGYVTNKGRQKVVPLTNTTNQKTELQAIYLALQDSGLEVNIVTNSQ
YALGIIQAQPDKSESELVNQIIEQLIKKEKVYLAWVPAHKGIGGNEQVDK
LVSA

3D structure

• PDB: 7lsk Structural basis of HIV inhibition by L-nucleosides: Opportunities for drug development and repurposing.
• Chain: A
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.-
  2.7.7.49: RNA-directed DNA polymerase.
  2.7.7.7: DNA-directed DNA polymerase.
  3.1.-.-
  3.1.13.2: exoribonuclease H.
  3.1.26.13: retroviral ribonuclease H.
  3.4.23.16: HIV-1 retropepsin.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	W24 F61 L74 D76 R78 N81 E89 I94 G152 W153 K154 P157 Y183 D185 D186 M230 G231 Q258 K259 G262 K263 W266 S280 R284 G285 K353 G359 H361 R448 T473 Q475 K476 Y501	W24 F61 L74 D76 R78 N81 E89 I94 G152 W153 K154 P157 Y183 D185 D186 M230 G231 Q258 K259 G262 K263 W266 S280 R284 G285 K353 G359 H361 R448 T473 Q475 K476 Y501
BS02	CA	A	D110 V111 G112 D185	D110 V111 G112 D185
BS03	XRF	A	K65 R72 D110 D113 A114 Y115 Q151 D185	K65 R72 D110 D113 A114 Y115 Q151 D185

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0004523 RNA-DNA hybrid ribonuclease activity

Biological Process

• GO:0006278 RNA-templated DNA biosynthetic process

External links

• PDB: RCSB:7lsk, PDBe:7lsk, PDBj:7lsk
• PDBsum: 7lsk
• PubMed: 35218925
• UniProt: P03366|POL_HV1B1 Gag-Pol polyprotein (Gene Name=gag-pol)