Structure of PDB 7lo5 Chain A

Receptor sequence

>7lo5A (length=1016) Species: 980427 (Deinococcus wulumuqiensis)

SLQLVKKFQKRLEDIVAYGGTRNESSVRAAFQQLLSDWAEGSGLRLITEV
TQKAVAGNNVRPDGTLKDSLQQSRGYWESKDEADTLDDEIQKKLAKGYPR
DNIIFEDSRLAVLMQNGEEVQRVDMGDAGALAGLLKLFFEFEPPQVLEFR
KAVDHFKDEMPHLLKILREAADAAEQKADYRGERDHFVEIAKEAINPDFS
PRDAREMLIQHILTGDLFTSVFDNAQYHEDNNIAQQLQQLAATFYKGPVK
RDIAERTKRYYGAIQAAAAQIADHHEKQRFLKALYENFYRAYNPAGAERL
GIFYTPGEIVRFMIEATDTLLEKHFQKELADKGVEILDPATGTGTFITEL
IDFLPKAKLEQKYREELHCNELALLPYYIANLNIEATYAQKMGRYEEFRN
IVLVDTLDNTGFSGLFGSVTAENLERAKRQNARPVRVIIGNPPYRANQAN
ENDNNKNREYKEIDRRIKATYVAASTAQKTKLYDMYSRFLRWATDRLKED
GIVAFVSNSSFIDSRTFDGFRKEVVKDFDHIYILDMKGNANTSGERRKRE
GGNVFNDQIKVGVAVYFLVRSAKDTKIWYHAVPDFWRAREKLEWLKTTKF
EDIEFDHIRPDAKHNWLGQVDEENDWNEFLPVADKDTKQAKGLGQERAIF
KLYSLGVVTNRDEWVYSRAEDELADKVRYFIGRYNEIIKLPLGDLMSRNW
EGDIKMTRATIADAQSRKSYSLEKNSIVPSLYRPFDVLKMYFSKNLNEMQ
YQMPSIFPKGVGENVVIALSGSPAAKPFQVLATDILPSLDLLEKTQCLPF
YRYTMNGERLNNITDYALKAFQTHYADTSISREDIFHYVYAVLHHPAYRE
KYALNLRQEFPRIPFYPEFGRWAAWGRELMALHIGFESVAPYPLKRTDEP
PKNDTPEALALAKKARLKVQRDAAKQPTGAVELDGLTTLAGIPAAAWAYK
LGNRSALEWVLERHKETTPKDATIREKFNTYRFADHKERVIDLLARVTTV
SVETVRIVGEMPAETM

3D structure

• PDB: 7lo5 Coordination of phage genome degradation versus host genome protection by a bifunctional restriction-modification enzyme visualized by CryoEM.
• Chain: A
• Resolution: 2.86 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	F304 N448 Y451 A484 Q485 K486 K488 N515 S521 R522 T523 F562 Q565 I566 K567 V568 L668 M762 Y764 Q765 P786 K807	F303 N441 Y444 A477 Q478 K479 K481 N508 S514 R515 T516 F555 Q558 I559 K560 V561 L655 M749 Y751 Q752 P773 K794
BS02	dna	A	N454 Q455 N457 E458 K463 Q485 K486 T487 Y490 A547 N548 R554 K567 R600 K651 V671 N673 D675 R721 R746 D803	N447 Q448 N450 E451 K456 Q478 K479 T480 Y483 A540 N541 R547 K560 R587 K638 V658 N660 D662 R708 R733 D790
BS03	dna	A	E25 S26 N60 R62 Y99	E24 S25 N59 R61 Y98
BS04	SAM	A	Y286 G302 F304 T306 A341 T342 T346 F347 E372 L373 D406 N448	Y285 G301 F303 T305 A340 T341 T345 F346 E371 L372 D405 N441
BS05	CA	A	E25 D64 E79 S80	E24 D63 E78 S79

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0004519 endonuclease activity
• GO:0008168 methyltransferase activity
• GO:0008170 N-methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:7lo5, PDBe:7lo5, PDBj:7lo5
• PDBsum: 7lo5
• PubMed: 33826880
• UniProt: A0A345IJ72