Structure of PDB 7lnj Chain A

Receptor sequence

>7lnjA (length=543) Species: 272563 (Clostridioides difficile 630)

ASGIYYTPKIIVDYIVKKTLKNHDIIKNPYPRILDISCGCGNFLLEVYDI
LYDLFEENIYELKKKYDENYWTVDNIHRHILNYCIYGADIDEKAISILKD
SLTNKKIKINLFCCDSLKKKWRYKFDYIVGNPPYIGHKKLEKKYKKFLLE
KYSEVYKDKADLYFCFYKKIIDILKQGGIGSVITPRYFLESLSGKDLREY
IKSNVNVQEIVDFLGANIFKNIGVSSCILTFDKKKTKETYIDVFKIKNED
ICINKFETLEELLKSSKFEHFNINQRLLSDEWILVNKDDETFYNKIQEKC
KYSLEDIAISFQGIITGCDKAFILSKDDVKLNLVDDKFLKCWIKSKNINK
YIVDKSEYRLIYSNDIDNENTNKRILDEIIGLYKTKLENRRECKSGIRKW
YELQWGREKLFFERKKIMYPYKSNENRFAIDYDNNFSSADVYSFFIKEEY
LDKFSYEYLVGILNSSVYDKYFKITAKKMSKNIYDYYPNKVMKIRIFRDN
NYEEIENLSKQIISILLNKSIDKGKVEKLQIKMDNLIMDSLGI

3D structure

• PDB: 7lnj Clostridioides difficile specific DNA adenine methyltransferase CamA squeezes and flips adenine out of DNA helix.
• Chain: A
• Resolution: 2.68 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	H171 K172 K176 K179 D192 K193 I349 G351 D353 K378 S379 K380 R425 Q438 W439 Y455 K456 I517	H137 K138 K142 K145 D158 K159 I315 G317 D319 K344 S345 K346 R391 Q404 W405 Y421 K422 I483
BS02	dna	A	Y30 N165 P166 Y168 H171 K173 K193 Y221 S227 F253 I256 G257 Q346 W439 R441 K456 Y476 K511 M513 S514 Y521 P522 N523	Y5 N131 P132 Y134 H137 K139 K159 Y187 S193 F219 I222 G223 Q312 W405 R407 K422 Y442 K477 M479 S480 Y487 P488 N489

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0032259 methylation

External links

• PDB: RCSB:7lnj, PDBe:7lnj, PDBj:7lnj
• PDBsum: 7lnj
• PubMed: 34103525
• UniProt: Q183J3