Structure of PDB 7lma Chain A

Receptor sequence

>7lmaA (length=1012) Species: 5911 (Tetrahymena thermophila)

KQMLTRKEDLLTVLKQISALKYVSNLYEFLLATEKIVQTSELDTQFQEFL
TTTIIASEQNLVENYKQKYNQPNFSQLTIKQVIDDSIILLGNKQNYVQQI
GTTTIGFYVEYENINLSRQTLYSSNFRNLLNIFGEEDFKYFLIDFLVFTK
VEQNGYLQVAGVCLNQYFSENQYIYPEIQRSQIFYCNHMGREPGVFKSSF
FNYSEPQTIIKKTLLKEYQSKNFSCQEERDLFLEFTEKIVQNFHNINFNY
LLKKFCKLPENYQSLKSQVKQIVQSENKANQQSCENLFNSLYDTEISYKQ
ITNFLRQIIQNCVPNQLLGKKNFKVFLEKLYEFVQMKRFENQKVLDYICF
MDVFDVEWFVDLKNQKFTQKRKYISDKRKILGDLIVFIINKIVIPVLRYN
FYITEKHKEGSQIFYYRKPIWKLVSKLTIVKLEEENLEKVEEKLIPEDSF
QKYPQGKLRIIPKKGSFRPIMTFLRKDKQKNIKLNLNQILMDSQLVFRNL
KDMLGQKIGYSVFDNKQISEKFAQFIEKWKNKGRPQLYYVTLDIKKCYDS
IDQMKLLNFFNQSDLIQDTYFINKYLLFQRNKRPLLQIMDNINFPYYFNL
KERQIAYSLYDDDDQILQKGFKEIQSDDRPFIVINQDKPRCITKDIIHNH
LKHISQYNVISFNKVKFRQKRGIPQGLNISGVLCSFYFGKLEEEYTQFLK
NAEQVNGSINLLMRLTDDYLFISDSQQNALNLIVQLQNCANNNGFMFNDQ
KITTNFQFPQEDYNLEHFKISVQNECQWIGKSIDMNTLEIKSIQKQTQQE
INQTINVAISIKNLKSQLKNKLRSLFLNQLIDYFNPNINSFEGLCRQLYH
HSKATVMKFYPFMTKLFQIDLKKSKQYSVQYGKENTNENFLKDILYYTVE
DVCKILCYLQFEDEINSNIKEIFKNLYSWIMWDIIVSYLKKKKQFKGYLN
KLLQKIRKSRFFYLKEGCKSLQLILSQQKYQLNKKELEAIEFIDLNNLIQ
DIKTLIPKISAK

3D structure

• PDB: 7lma Structures of telomerase at several steps of telomere repeat synthesis.
• Chain: A
• Resolution: 3.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.49: RNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	rna	A	K11 Q218 Y221 H234 M235 R237 P239 F242 K243 S244 N324 Y325 K328 L333 Y337 Q338 K341 K374 K395 L420 C424 F429 K445 R446 I449 S450 R453 R473 R534 L549 R550 K551 D552 K553 K576 N656 K657 R658 P693 Y694 N697 L775 N776 I909 K913 K917 R921 I967 K971 S972 K973 V977 Y994 Y995 E1019 K1038 K1040 K1041 Q1052 K1056 S1057 F1059 F1060 Y1061 K1063 K1101	K1 Q172 Y175 H188 M189 R191 P193 F196 K197 S198 N249 Y250 K253 L258 Y262 Q263 K266 K299 K320 L345 C349 F354 K370 R371 I374 S375 R378 R398 R459 L474 R475 K476 D477 K478 K501 N581 K582 R583 P595 Y596 N599 L677 N678 I811 K815 K819 R823 I869 K873 S874 K875 V879 Y896 Y897 E921 K940 K942 K943 Q954 K958 S959 F961 F962 Y963 K965 K1003
BS02	dna	A	F414 I877 T902 K919 K956	F339 I779 T804 K821 K858

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003720 telomerase activity
• GO:0003723 RNA binding
• GO:0003964 RNA-directed DNA polymerase activity
• GO:0042162 telomeric DNA binding
• GO:0046872 metal ion binding
• GO:0070034 telomerase RNA binding

Biological Process

• GO:0007004 telomere maintenance via telomerase

Cellular Component

• GO:0000333 telomerase catalytic core complex
• GO:0000781 chromosome, telomeric region
• GO:0005634 nucleus
• GO:0005694 chromosome
• GO:0005697 telomerase holoenzyme complex
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:7lma, PDBe:7lma, PDBj:7lma
• PDBsum: 7lma
• PubMed: 33981033
• UniProt: O77448|TERT_TETTS Telomerase reverse transcriptase (Gene Name=TERT)