Structure of PDB 7lkz Chain A

Receptor sequence

>7lkzA (length=1920) Species: 9606 (Homo sapiens)

FVRQIQLLLWKNWTLRKRQKIRFVVELVWPLSLFLVLIWLRNANPLYSHH
ECHFPNKAMPSAGMLPWLQGIFCNVNNPCFQSPTPGESPGIVSNYNNSIL
ARVYRDFQELLMNAPESQHLGRIWTELHILSQFMDWGGILSDMSPRIQEF
IHRPSMQDLLWVTRPLGPETFTKLMGILSDLLCGYPEGGGWYEDNNYKAF
LGYDRRTTSFCNALIQSLESNPLTKIAWRAAKPLLMGKILYTPDSPAARR
ILKNANSTFEELEHVRKLVKAWEEVGPQIWYFFDNSTQMNMIRDTLGNPT
VKDFLNRQLDWRDIFNITDRTLRLVNQYLECLVLDKFESYNDETQLTQRA
LSLLEENMFWAGVVFPDMYPWTSSLPPHVKYKIRMDIDVVEKTNKIKDRY
WDSGPRADPVEDFRYIWGGFAYLQDMVEQGITRSQVQAEAPVGIYLQQMP
YPCFVDDSFMIILNRCFPIFMVLAWIYSVSMTVKSIVLEKELRLKETLKN
QGVSNAVIWCTWFLDSFSIMSMSIFLLTIFIMHGRILHYSDPFILFLFLL
AFSTATIMLCFLLSTFFSKASLAAACSGVIYFTLYLPHILCFAWQDRMTA
ELKKAVSLLSPVAFGFGTEYLVRFEEQGLGLQWSNIGNSPTEGDEFSFLL
SMQMMLLDAAVYGLLAWYLDQVFPGDYGTPLPWYFLLQESYWLHDSFFER
EHPGWVPGVCVKNLVKIFEPCGRPAVDRLNITFYENQITAFLGHNGAGKT
TTLSILTGLLPPTSGTVLVGGRDIETSLDAVRQSLGMCPQHNILFHHLTV
AEHMLFYAQLKGKSQEEAQLEMEAMLEDTGLHHKRNEEAQDLSGGMQRKL
SVAIAFVGDAKVVILDQPTSGVDPYSRRSIWDLLLKYRSGRTIIMSTHHM
DEADLLGDRIAIIAQGRLYCSGTPLFLKNCFGTGLYLTLVRKMKQVLDGD
VNELMDVVLHHVPEAKLVECIGQELIFLLPNKNFKHRAYASLFRELEETL
ADLGLSSFGISDTPLEEIFLKVTEDSPQLNTGTQLVLQHVQALLVKRFQH
TIRSHKDFLAQIVLPATFVFLALMLSIVIPPFGEYPALTLHPWIYGQQYT
FFSMDEPGSEQFTVLADVLLNKPGFGNRCLKEGWLPEYPCGNSTPWKTPS
VSPNITQLFQKQKWTQVNPSPSCRCSTREKLTMLPECPEGAGGLPPPQRT
QRSTEILQDLTDRNISDFLVKTYPALIRSSLKSKFWVNEQRYGGISIGGK
LPVVPITGEALVGFLSDLGRIMNVSGGPITREASKEIPDFLKHLETEDNI
KVWFNNKGWHALVSFLNVAHNAILRASLPKDRSPEEYGITVISQPLNLTK
EQLSEITVLTTSVDAVVAICVIFSMSFVPASFVLYLIQERVNKSKHLQFI
SGVSPTTYWVTNFLWDIMNYSVSAGLVVGIFIGFQKKAYTSPENLPALVA
LLLLYGWAVIPMMYPASFLFDVPSTAYVALSCANLFIGINSSAITFILEL
FENNRTLLRFNAVLRKLLIVFPHFCLGRGLIDLALSQAVTDVYARFGEEH
SANPFHWDLIGKNLFAMVVEGVVYFLLTLLVQRHFFLDEDDDVAEERQRI
ITGGNKTDILRLHELTKIYPGTSSPAVDRLCVGVRPGECFGLLGVNGAGK
TTTFKMLTGDTTVTSGDATVAGKSILTNISEVHQNMGYCPQFDAIDELLT
GREHLYLYARLRGVPAEEIEKVANWSIKSLGLTVYADCLAGTYSGGNKRK
LSTAIALIGCPPLVLLDQPTTGMDPQARRMLWNVIVSIIREGRAVVLTSH
SMEECEALCTRLAIMVKGAFRCMGTIQHLKSKFGDGYIVTMKIKSLLPDL
NPVEQFFQGNFPGSVQRERHYNMLQFQVSSSSLARIFQLLLSHKDSLLIE
EYSVTQTTLDQVFVNFAKQQ

3D structure

• PDB: 7lkz Molecular structures of the eukaryotic retinal importer ABCA4.
• Chain: A
• Resolution: 3.27 Å

Enzymatic activity

• Enzyme Commision number: 7.6.2.1: P-type phospholipid transporter.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	6OU	A	R653 F780 A781 Q783 S1391 E1670 S1677	R465 F592 A593 Q595 S1076 E1355 S1362
BS02	6OU	A	F754 F755 S756 L760	F566 F567 S568 L572
BS03	6OU	A	R24 F1785 D1786	R22 F1470 D1471
BS04	MG	A	T970 Q1010	T750 Q790
BS05	ATP	A	F938 A945 N965 G966 G968 K969 T970 T2070 S2072 G2073	F718 A725 N745 G746 G748 K749 T750 T1742 S1744 G1745
BS06	ATP	A	K1054 D1061 S1063 Y1947 N1974 G1975 G1977 K1978 T1979 T1980	K834 D841 S843 Y1619 N1646 G1647 G1649 K1650 T1651 T1652

Gene Ontology

Molecular Function

• GO:0003924 GTPase activity
• GO:0005501 retinoid binding
• GO:0005502 11-cis retinal binding
• GO:0005503 all-trans retinal binding
• GO:0005524 ATP binding
• GO:0005548 phospholipid transporter activity
• GO:0016887 ATP hydrolysis activity
• GO:0034632 retinol transmembrane transporter activity
• GO:0042626 ATPase-coupled transmembrane transporter activity
• GO:0090555 phosphatidylethanolamine flippase activity
• GO:0140326 ATPase-coupled intramembrane lipid transporter activity
• GO:0140327 flippase activity
• GO:0140347 N-retinylidene-phosphatidylethanolamine flippase activity
• GO:0140359 ABC-type transporter activity

Biological Process

• GO:0001523 retinoid metabolic process
• GO:0006649 phospholipid transfer to membrane
• GO:0006869 lipid transport
• GO:0007601 visual perception
• GO:0007603 phototransduction, visible light
• GO:0034633 retinol transport
• GO:0042574 retinal metabolic process
• GO:0045332 phospholipid translocation
• GO:0045494 photoreceptor cell maintenance
• GO:0055085 transmembrane transport

Cellular Component

• GO:0001750 photoreceptor outer segment
• GO:0005783 endoplasmic reticulum
• GO:0005886 plasma membrane
• GO:0016020 membrane
• GO:0031410 cytoplasmic vesicle
• GO:0042995 cell projection
• GO:0043231 intracellular membrane-bounded organelle
• GO:0097381 photoreceptor disc membrane
• GO:0120202 rod photoreceptor disc membrane

External links

• PDB: RCSB:7lkz, PDBe:7lkz, PDBj:7lkz
• PDBsum: 7lkz
• PubMed: 33605212
• UniProt: P78363|ABCA4_HUMAN Retinal-specific phospholipid-transporting ATPase ABCA4 (Gene Name=ABCA4)