Structure of PDB 7l35 Chain A

Receptor sequence

>7l35A (length=634) Species: 9606 (Homo sapiens)

SNDPSGYNPAKNNYHPVEDACWKPGQKVPYLAVARTFEKIEEVSARLRMV
ETLSNLLRSVVALSPPDLLPVLYLSLNHLGPPQQGLELGVGDGVLLKAVA
QATGRQLESVRAEAAEKGDVGLVAENSPPPPLTASGVFSKFRDIARLTGS
ASTAKKIDIIKGLFVACRHSEARFIARSLSGRLRLGLAEQSVLAALSQAV
SLTPPGQEFPPAMVDAGKGKTAEARKTWLEEQGMILKQTFCEVPDLDRII
PVLLEHGLERLPEHCKLSPGIPLKPMLAHPTRGISEVLKRFEEAAFTCEY
KYDGQRAQIHALEGGEVKIFSRNQEDNTGKYPDIISRIPKIKLPSVTSFI
LDTEAVAWDREKKQIQPFQVLTTRKRKEVDASEIQVQVCLYAFDLIYLNG
ESLVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKD
SCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLG
RGKWAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVL
PSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKG
ISLRFPRFIRVREDKQPEQATTSAQVACLYRKQS

3D structure

• PDB: 7l35 LIG1 syndrome mutations remodel a cooperative network of ligand binding interactions to compromise ligation efficiency.
• Chain: A
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 6.5.1.1: DNA ligase (ATP).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L347 G348 V349 G350 G352 V353 G571 R573 S588 E592 F635 F872	L88 G89 V90 G91 G93 V94 G304 R306 S321 E325 F368 F605
BS02	dna	A	S303 K746 Y749 T798 G799 F800 S801 D802 F872 R874	S44 K479 Y482 T531 G532 F533 S534 D535 F605 R607
BS03	dna	A	R305 T415 G416 S417 A418 T420 R451 G453 A455 Q457 S458 Q636 T639 T640 R641 R643 R738 R768 G776 K795 G797 S850 L851 S852 P853 Y855 S869 L870 F872	R46 T148 G149 S150 A151 T153 R184 G186 A188 Q190 S191 Q369 T372 T373 R374 R376 R471 R501 G509 K528 G530 S583 L584 S585 P586 Y588 S602 L603 F605
BS04	AMP	A	K568 Y569 R573 E621 F660 M723 K725 K744 K746	K301 Y302 R306 E354 F393 M456 K458 K477 K479

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003909 DNA ligase activity
• GO:0003910 DNA ligase (ATP) activity
• GO:0005524 ATP binding

Biological Process

• GO:0006281 DNA repair
• GO:0006310 DNA recombination
• GO:0071897 DNA biosynthetic process

External links

• PDB: RCSB:7l35, PDBe:7l35, PDBj:7l35
• PDBsum: 7l35
• PubMed: 33444456
• UniProt: P18858|DNLI1_HUMAN DNA ligase 1 (Gene Name=LIG1)