Structure of PDB 7kt1 Chain A

Receptor sequence

>7kt1A (length=328) Species: 9606 (Homo sapiens)

WMPAYACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVL
KALPSPVTTLSQLQGLPHFGEHSSRVVQELLEHGVCEEVERVRRSERYQT
MKLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGLQHHQDL
STPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLIT
HPKEGQEAGLLPRVMCRLQDQGLILYHQHAFERSFCIFRLPQPGSWKAVR
VDLVVAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQ
KTFFQAASEEDIFRHLGLEYLPPEQRNA

3D structure

• PDB: 7kt1 Watching a double strand break repair polymerase insert a pro-mutagenic oxidized nucleotide.
• Chain: A
• Resolution: 1.665 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L177 F385 E386 R387 R442 R445 R449 K450 L456 N457	L41 F231 E232 R233 R276 R279 R283 K284 L290 N291
BS02	dna	A	G245 V246 G247 T250 D330 D332 F389 R416 D418 G433 W434 T435 G436 K438 R445	G109 V110 G111 T114 D194 D196 F235 R250 D252 G267 W268 T269 G270 K272 R279
BS03	dna	A	G174 R175 T178 F179 P203 H204 G206 H208 S209	G38 R39 T42 F43 P67 H68 G70 H72 S73
BS04	DGT	A	G320 R323 H329 D330 D332 G433 W434 T435 G436 K438 R445	G184 R187 H193 D194 D196 G267 W268 T269 G270 K272 R279
BS05	MN	A	D330 D332 D418	D194 D196 D252
BS06	MN	A	D330 D332	D194 D196
BS07	MN	A	E386 H459	E232 H293
BS08	MN	A	E218 H219	E82 H83

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:7kt1, PDBe:7kt1, PDBj:7kt1
• PDBsum: 7kt1
• PubMed: 33824325
• UniProt: Q9NP87|DPOLM_HUMAN DNA-directed DNA/RNA polymerase mu (Gene Name=POLM)