Structure of PDB 7ksv Chain A

Receptor sequence

>7ksvA (length=325) Species: 9606 (Homo sapiens)

MPAYACQRPTPLTHHNTGLSEALEILAEAAGFEGSEGRLLTFCRAASVLK
ALPSPVTTLSQLQGLPHFGEHSSRVVQELLEHGVCEEVERVRRSERYQTM
KLFTQIFGVGVKTADRWYREGLRTLDDLREQPQKLTQQQKAGLQHHQDLS
TPVLRSDVDALQQVVEEAVGQALPGATVTLTGGFRRGKLQGHDVDFLITH
PKEGQEAGLLPRVMCRLQDQGLILYHQFERSFCIFRLPQPGSWKAVRVDL
VVAPVSQFPFALLGWTGSKLFQRELRRFSRKEKGLWLNSHGLFDPEQKTF
FQAASEEDIFRHLGLEYLPPEQRNA

3D structure

• PDB: 7ksv Watching a double strand break repair polymerase insert a pro-mutagenic oxidized nucleotide.
• Chain: A
• Resolution: 1.64 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	A	L177 F385 E386 R387 R442 R445 R449 K450 L456 N457	L40 F228 E229 R230 R273 R276 R280 K281 L287 N288
BS02	dna	A	G245 V246 G247 T250 D330 D332 F389 R416 D418 G433 W434 T435 G436 K438 R445	G108 V109 G110 T113 D193 D195 F232 R247 D249 G264 W265 T266 G267 K269 R276
BS03	dna	A	G174 R175 T178 F179 H204 G206 H208 S209	G37 R38 T41 F42 H67 G69 H71 S72
BS04	MN	A	D330 D332 D418	D193 D195 D249
BS05	MN	A	D330 D332	D193 D195
BS06	MN	A	E215 H219	E78 H82
BS07	MN	A	E386 H459	E229 H290
BS08	GOA	A	G319 G320 R323	G182 G183 R186

Gene Ontology

Molecular Function

• GO:0003677 DNA binding
• GO:0003887 DNA-directed DNA polymerase activity
• GO:0016779 nucleotidyltransferase activity
• GO:0034061 DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

Cellular Component

• GO:0005634 nucleus

External links

• PDB: RCSB:7ksv, PDBe:7ksv, PDBj:7ksv
• PDBsum: 7ksv
• PubMed: 33824325
• UniProt: Q9NP87|DPOLM_HUMAN DNA-directed DNA/RNA polymerase mu (Gene Name=POLM)