Structure of PDB 7k5b Chain A

Receptor sequence

>7k5bA (length=4453) Species: 5911 (Tetrahymena thermophila)

KYSKRIAWMKTTICDSLQLKDMIVEESFQYEKNKNLLEQFLSGEGLNKIF
AYYQVQEQAQNTTGDLEKIQDKAVWFLRITNPADDKKKASQQDGNDNDII
FGEITPNTVPMLNALMESVYSRQIDHIITEKIQFWGVAEEEQVLEFQQHS
NKFSSEVREAINLDYEAISGLSESEKMQHYEMKFNEWINLISSQLRKDEK
DAGPATELIYWRSRMQKITNWSEQLKSKDFQIVKASLQRHKNHDNQRPRG
DESKLNEAKDNVKYLTTLEKFIPLYNGTPQQIIDTLPALMNAIKMIHTTT
DKMTGLFIKITNQMIKNCKDRILNKMIWEQDPAELIEVLGSCIKLYCEYK
KCYNDTKEKVADMPKGKTSDAQIFGKFDTFVRRLQKLIEIFSNIQQFNAL
LEGMDVLTNKFKKIIDDFKKKGHNLLDTANNKFDRDWVEFNVEISHLDGE
LQNFIDNNFNRFRYSLKLLHKFQSTIKRHNLTSRYNAILHNYATELDTIQ
RVFQDQKSNPPLVRNMPPEAGKIIWARHLFQKITGPINIFPTEIRRYYGS
YNTLGKQLTIYEMWFYQDWVNKIEQSKAALQATLIVRHDENKKLYVNFDL
EIMQLIREAKCLDRQGIEIPESARIILLQEDKFKTYYNELLYALKEYERI
NSKIKPICKNLLLPHIEDLDLKLRPGMVTLTWTSMNIESYLYYVHQGLKK
LEQLIINVNDIIENRIENNLKTVSKVVLVHLPLDSFVQLQEEYINSKTDF
LTSKNVEVERAVDDLLQTIMLYPLDPHVDPVLPEETKRIKRYYFWYFYQA
LLNSTQNSLNAMKYRVFFQVEVQLNGDKVTLNLQEIQKSINRAATAVLRC
SKHLYNWDQATFYDMIACDKEIVKVILLLTGSIQGTKNKVNEFLSGFTKF
EWLCKESIQESIKKFSKNGPTLQNYEDQLKKFSQIEEEIEKIVPTYKIGA
MELMTHNICTSLSTWAKEWKLQYSQDLHKRARQLLDSLTEQTKMLSTKLS
KPVKDIDSLGYVMETLEQIRKEQAEIDMKFNPVQEMYSLLDNYLPGGITD
KDEMDARSLLRRNWDILIQQAEIKGKEYQHKQAIYLKELKQSIKDFTNQV
SIFRRDYEKNGPMERLRRFEDEYDVKYQMYKINARGENLFGLQNQKYPEL
EKTDAEIKNLNKLYNLYDSVIKNIQQFKEKSWQDVSLAKMEEDAGKYGEQ
CSRLPKDLKEWQAYRDLKNYIDSLREQLPLIIPRHWEKIKEITNTKLNYE
NPDQFYIEEIMGAKLDFREDIEDITESADKQLKIRTGLDEINLYWNDMQF
QFGIWGKRMLNGLIVGTILERLEEDQLQLSTFNSQRHVTPFKAEVENLIR
TFSDVNDTLDMWVKVQKLWTSLEPVFTGGDIARQMPLQAKQFQGIDKNWM
KIMEKAVETKKVIPCCQNDMLKDFLPDLNRKLEDCQKMLEAYLEGKRKKF
PRFYFVSNPTLLKILSQGSEPTSIQEDFEKLFDAITKVTFESAKDKKNPA
LKQITQIQQVIGRNEENISLTGYYVKCEGNIEDWLKKLEQNMQQTLKDIA
SAAAQQVFQVGLKEFVSSQASQIALLGLQILWTSKVNEGLERLSRNERNA
MDIKRNEIKEHMNILSSMCLEDLNGAVERTKVETLVTIQVHQKDISMDLK
CKDVNDFEWQKQTRIAWKTDIDECIISITDWDSPYSYEFLGAKERLCITP
LTDRCYITLAQAMSMYYGGAPAGPAGTGKTETVKDLGRTLGVFVVVTNCS
DQHRYRDMAKIFKGLVQSGLWGCFDEFNRIDLEVLSVVAMQVESITTARK
QHMKKFMFPEEEIEIELIPTVSYFITMNPGYAGRQELPENLKVLFRGVSM
MVPDREIIIKVKLASVGYLQIDLLAKKFNVLYRLCEEQLSKQRHYDFGLR
NILSVLRTAGNTKRQEIKSDEEMLLMRSLRDMNLSKLVADDIPLFNGLLA
DIFPKLKEVPKKLYPDVEKKIPEEINAESYLINTPSFQLKIIQLYETCLV
RHGFMLVGPTGSGKSTIMKILTEVLTKLGSPHKIVIMNPKAITAEQMYGV
KSEISDDWIPGVFSTIWAKSNNRALKHTTWITCDGPVDAIWIENLNTVLD
DNKILTLANGERIAMTENCKVVFEVENLNNASPATVSRCGQVYVSPTDLG
YEAVIEGWIRNRKASGRAEESDKLGNILRKYLINMRFIELQSKECKEPMM
DTSPVISVINILNLLTGCLQYFVQTQRTLSEQEYEKFIVYSMAWAIGGIY
EAQDRVRFHELLLAKNAPIPQKGKENETVFDYYVSQDYLDWKICSPEEWV
PPQSLQFSQLLLPTLDSFRAEMLLNFILTQPKSHTCSNSALLIGGSGTAK
TSSVLLYCNKFDPQKMLFKRTNFSSATSPFMFQSTIEAECDFKVGKEFAP
PGNKMMTIFIDDMSMPFVNKWGDQITLELVRQLIETGGFYMLDKTQRGNQ
RKMKNLQYIGAMNHPGGGRNDIPNRLKRQFFIFNMILPLSIEGIYGPIIK
HMFKQKYFSDSTYKVIESLTSATIALWNKVKSTMLPTPAKFHYVFNMREL
SRIFKGILTCKKDTINDAPKSMKIKPELFLVGLWRHEAERVLADKLVNNK
DKDTVMGYIQEVSLESFSQIENEILEKYSSEKTFLFCDFLRPDVINEDGI
IEEEAPKIYEAIDSLTELRKRCNFLLSFYNDRNPSKKMPLVLFDDALKHL
LRISRIIRQPRSSGLLVGVGGSGKQSLTRLAGFIGKNLIQQIIVTKTYSD
KDLKEDIKKGFDDAGHLGKQVTFLMTDSEVKKEEFLEYINMVLSTGEIPN
LLAKDEREVWLGDISQAYCKEKNLGNIDPPQSELWTYFVDRVRDNFHIML
CFSPVGQKFRERARKFPALFNECTIDWFLPWPEEALVSVAETFIKNFDKL
DTKEETKQELMKHMGNVHLMVNEICDEYYQKMRRQVYVTPKSFLSYLNSY
KTLYIEKYDELDQQEESFKIGLNKIQEATITINQMEISLKEEEIQLNEAT
EKTNQLLANLDKESKKANQKGEEVAATNKQCEIQAEQISKEKEEAERELE
AALPALRRAQEAVDSIESKDIVELKANKKPLDIIKYIMNAVLVFFKARLI
PIQIEERVFNKKEGKAVLFLKESYDESGIQTLGDMNFMKKLKEFEKDSIN
EETIELLEPYLNQSEDWFNDTFATKASKAAAGILKWAFAIYEYHQKSKIV
KPKRIQVAIAEGRQAIALKELEKAREDLAQIQAYIKNLKDVYTKQMEEKN
ELEMKAAKTKKKINTARTLITSLSGEKDRWGKGAQDISDQKRKLVGNVSL
STAFISYCGPFNAEYRNKLAQQRFVVDMKKRGVPVTPGLELTSFLIDDAT
IGEWNLQGLPKDDLSIQNGIMVTNSARYPLFIDPQGQGQNWIRNKLSASI
IPERCITTLSHPKFKDMFLKYCMESGLTLIVENIENEVDPMMDPVLERQI
IVKGKTQFVNVAGTEMELSKEFKLFMTCRLANPSFSPELSAKTTIIDFTV
TQSGLEQQLLGKVISKEQKALEDSLNQLLADVNQNQKDLQRLDKNLLERL
INSQGNLLDDTELMDVLNNTKTQAKEVAAKLIDAEIKTKEINEKREQYRP
VAIRGSAIYFTMIEVSLVNWMYNSSLEQFLKLFIESIDLSEKAQLPSNRV
KNIISFLTFHVYRYVNRGLFEKDKITFILMMAFKILTTAGTISSGDVSLF
LKSGDALDIKSERQKQISYLEDNQWLNILALSKHTFSGQTLPFFKELPDL
ISRSENQWRNWIDKNDPENFPIPDFAESINQEKEIGSFISLCLVRSLRND
RTLIATQNFISNVLGKEFTDPISYPIEGIWQESSNMDPVLFLLSAGADPT
SSIDELAKKKKKFPCEKVSMGEGQERVARQVIMKGFVEGGWVILQNCHLG
LKFMEEIETLVSPINQIHEDFRLWITCEQHPKFPLGLLQKTLKVTNEPPK
GLKAGLYKTFTTIITQEFIDKVDHSNWRSLIFTICFLHSIVIERKKFGPL
GWCVPYEYNYSDLEASLLYIEKYLTNLMSTPHNLPISMNVVRYMICEVQY
GGRITDDLDRELFITYGETYLKDGIFGNDYFFYDIMVDQKFKYRIPQNPS
AELIKYQEYIAKVPTVDNPEVFGLHSNADLTFRLKESKEMINTVMETRPK
DSSVGGGKTREEIVQDKAKDMLKNLPPDYNDVEVRELVSKLGGPNPKTST
ERGMTVPLNIFLYQEVTRMQRVIGLVRKTLQDTILAIDGQIIMTPEILEA
INAIYDAKVPNSWLYDPSGAEISWLLPNLGSWSTSLSDRNKQLNDWLRSG
QRPILFWLTGFFNPQGFLTGMKQEVTRNHKGKGGEAWSLDDVVYSTTVKE
REKEKDIEQPPAEGVYIKGLYLEGCKWSKNGLDDSDPKKIFADLPILHVS
AINKKMSNTYLCPVYKYPKRTDKYLIFRVGLPCEGSNNPSHWKLRGVALL
CST

3D structure

• PDB: 7k5b Structures of outer-arm dynein array on microtubule doublet reveal a motor coordination mechanism.
• Chain: A
• Resolution: 4.5 Å

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ADP	A	L2460 L2461 T2463 G2496 T2497 A2498 K2499 T2500 S2501 M2696 R2697	L2311 L2312 T2314 G2347 T2348 A2349 K2350 T2351 S2352 M2547 R2548
BS02	ATP	A	Y2129 L2130 I2131 F2136 G2160 G2162 K2163 S2164 T2165 E2273 L2298 V2303 H2483 R2624 R2627	Y1980 L1981 I1982 F1987 G2011 G2013 K2014 S2015 T2016 E2124 L2149 V2154 H2334 R2475 R2478
BS03	ADP	A	L2839 V2840 F2842 G2870 S2871 G2872 K2873 Q2874 S2875 W3030 L3093	L2690 V2691 F2693 G2721 S2722 G2723 K2724 Q2725 S2726 W2881 L2944
BS04	MG	A	S2164 R2627	S2015 R2478

Gene Ontology

Molecular Function

• GO:0005524 ATP binding
• GO:0008569 minus-end-directed microtubule motor activity
• GO:0016887 ATP hydrolysis activity
• GO:0045505 dynein intermediate chain binding
• GO:0051959 dynein light intermediate chain binding

Biological Process

• GO:0007018 microtubule-based movement

Cellular Component

• GO:0005737 cytoplasm
• GO:0005856 cytoskeleton
• GO:0005858 axonemal dynein complex
• GO:0005874 microtubule
• GO:0005929 cilium
• GO:0030286 dynein complex

External links

• PDB: RCSB:7k5b, PDBe:7k5b, PDBj:7k5b
• PDBsum: 7k5b
• PubMed: 34556869
• UniProt: Q22A67